Introgression of wilt resistance from wild into susceptible cultivated species of safflower

Abstract

Six wild species have been repeatedly confirmed for high resistance against Fusarium wilt in wilt-sick plots in glasshouse and wilt-sick plots. True-hybridity of F1 of the crosses, Nira (Carthamus tinctorius L.) x C. oxyacantha,Nira (Carthamus tinctorius L.) x C. palaestinus and Nira (Carthamus tinctorius L.) x C. lanatuswas confirmed with SSR markers. Marker-assisted selection was practiced for wilt resistance using SSR markers flanked to wilt resistance in F2 to F6 generations of (Nira x C. oxyacantha). Simultaneously phenotyping for reaction against wilt was done by growing parents and various generations (F2 to F6) in wilt sick plot. Wilt incidence in various generations has ranged from 0-7%. It indicated successful transfer of wilt resistance form resistant wild species, C. oxyacantha into wilt susceptible cultivated variety ‘Nira’ belonging to cultivated species (C. tinctorius). Marker-assisted selection was practiced for wilt resistance using SSR markers flanked to wilt resistance in F2 to F5 generations of (Nira x C. palaestinus) (Fig. 7). Simultaneously phenotyping for reaction against wilt was done by growing parents and various generations (F2 to F5) in wilt sick plot. Wilt incidence in various generations has ranged from 0-7%. It indicated successful transfer of wilt resistance form resistant wild species, C. palaestinus into wilt susceptible cultivated variety ‘Nira’ belonging to cultivated species (C. tinctorius) . BSA was done in F2 generations of crosses (Nira x C. lanatus) SSR markers flanked to wilt resistance in C.lanatus were identified. Markers flanked to wilt resistance were identified in the cross (Fig.9). MAS was practiced in F2 and simultaneously F2 was screened against wilt in wilt sick plot. The Markers were validated in F2.