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http://krishi.icar.gov.in/jspui/handle/123456789/29387
Title: | Development and evaluation of real-time RT-PCR using ear hair for specific detection of sheep persistently infected with border disease virus (BDV). |
Other Titles: | Not Available |
Authors: | Kalaiyarasu S Mishra N Rajukumar K Behera SP Jhade SK Singh VP |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Institute of High Security Animal Diseases |
Published/ Complete Date: | 2019-04-03 |
Project Code: | Not Available |
Keywords: | Border disease; Persistent infection; Hair follicle; Real time RT-PCR; Virus isolation |
Publisher: | Elsevier B.V. |
Citation: | Kalaiyarasu S, Mishra N, Rajukumar K, Behera SP, Jhade SK, Singh VP (2019) Development and evaluation of real-time RT-PCR using ear hair for specific detection of sheep persistently infected with border disease virus (BDV). Journal of Virological methods. 269:55-63. |
Series/Report no.: | Not Available; |
Abstract/Description: | The aim of this study was to develop an improved border disease virus (BDV) specific real time RT-PCR and to evaluate its performance on manually plucked hairs from sheep persistently infected with BDV that may act as a non-invasive alternate sample. The BDV real time RT-PCR assay reported here showed a high analytical sensitivity (100.6 TCID50/ml), specificity (no reactivity with BVDV-1, BVDV-2, HoBi-like pestivirus and CSFV) and reproducibility. When the assay was validated on 210 samples from BDV-infected and uninfected sheep, it showed a 100% diagnostic sensitivity and specificity with virus isolation. Further evaluation of the assay on manually plucked hair follicles from ear (mid-lateral, mid-medial) and tail tip from sheep persistently infected with BDV showed that a minimum of 20 hair follicles need to be tested for correct diagnosis of BDV. The BDV load was comparatively higher in hairs from mid-medial ear than those from other tested locations. Evaluation on other samples from PI sheep demonstrated that the test performance was similar to that of pestivirus generic real-time RT-PCR, but improved than the currently available BDV specific real-time RT-PCR. Although more number of PI animals need to be evaluated, the results of the study showed that manually plucked hairs from mid-medial ear pinna is a suitable alternative sample in real-time RT-PCR for detection of BDV persistently infected sheep. Use of the non-invasive ear hair samples and the improved BDV specific real-time RT-PCR reported here may be useful for BDV surveillance in several sheep rearing countries. |
Description: | Not Available |
ISSN: | 0166-0934 |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Virological Method |
NAAS Rating: | 7.79 |
Volume No.: | 269 |
Page Number: | 55-63 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1016/j.jviromet.2019.04.003 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/29387 |
Appears in Collections: | AS-NIHSAD-Publication |
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