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http://krishi.icar.gov.in/jspui/handle/123456789/29387
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kalaiyarasu S | en_US |
dc.contributor.author | Mishra N | en_US |
dc.contributor.author | Rajukumar K | en_US |
dc.contributor.author | Behera SP | en_US |
dc.contributor.author | Jhade SK | en_US |
dc.contributor.author | Singh VP | en_US |
dc.date.accessioned | 2020-01-08T06:27:48Z | - |
dc.date.available | 2020-01-08T06:27:48Z | - |
dc.date.issued | 2019-04-03 | - |
dc.identifier.citation | Kalaiyarasu S, Mishra N, Rajukumar K, Behera SP, Jhade SK, Singh VP (2019) Development and evaluation of real-time RT-PCR using ear hair for specific detection of sheep persistently infected with border disease virus (BDV). Journal of Virological methods. 269:55-63. | en_US |
dc.identifier.issn | 0166-0934 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/29387 | - |
dc.description | Not Available | en_US |
dc.description.abstract | The aim of this study was to develop an improved border disease virus (BDV) specific real time RT-PCR and to evaluate its performance on manually plucked hairs from sheep persistently infected with BDV that may act as a non-invasive alternate sample. The BDV real time RT-PCR assay reported here showed a high analytical sensitivity (100.6 TCID50/ml), specificity (no reactivity with BVDV-1, BVDV-2, HoBi-like pestivirus and CSFV) and reproducibility. When the assay was validated on 210 samples from BDV-infected and uninfected sheep, it showed a 100% diagnostic sensitivity and specificity with virus isolation. Further evaluation of the assay on manually plucked hair follicles from ear (mid-lateral, mid-medial) and tail tip from sheep persistently infected with BDV showed that a minimum of 20 hair follicles need to be tested for correct diagnosis of BDV. The BDV load was comparatively higher in hairs from mid-medial ear than those from other tested locations. Evaluation on other samples from PI sheep demonstrated that the test performance was similar to that of pestivirus generic real-time RT-PCR, but improved than the currently available BDV specific real-time RT-PCR. Although more number of PI animals need to be evaluated, the results of the study showed that manually plucked hairs from mid-medial ear pinna is a suitable alternative sample in real-time RT-PCR for detection of BDV persistently infected sheep. Use of the non-invasive ear hair samples and the improved BDV specific real-time RT-PCR reported here may be useful for BDV surveillance in several sheep rearing countries. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Elsevier B.V. | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Border disease; Persistent infection; Hair follicle; Real time RT-PCR; Virus isolation | en_US |
dc.title | Development and evaluation of real-time RT-PCR using ear hair for specific detection of sheep persistently infected with border disease virus (BDV). | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Article | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Journal of Virological Method | en_US |
dc.publication.volumeno | 269 | en_US |
dc.publication.pagenumber | 55-63 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://doi.org/10.1016/j.jviromet.2019.04.003 | en_US |
dc.publication.authorAffiliation | ICAR::National Institute of High Security Animal Diseases | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 7.79 | en_US |
Appears in Collections: | AS-NIHSAD-Publication |
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