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http://krishi.icar.gov.in/jspui/handle/123456789/3067| Title: | Cost effective SNP genotyping assays for castor |
| Project Code: | BT/CRS00013/CRS-01/12 |
| Published/ Complete Date: | 2016-12-18 |
| Division or Unit: | Crop Improvement |
| PI Name: | Senthilvel Senapathy |
| Project Investigator Designation: | Senior Scientist |
| Project Investigator Email: | senthilvel.senapathy@icar.gov.in |
| Current Location of Data : | PI |
| Current Person's Email: | senthilvel.senapathy@icar.gov.in |
| Since when: | 2016-12-18 |
| Abstract/Description: | A cost-effective SNP genotyping assay based on ‘Kompetitive Allele Specific PCR genotyping system (KASP), a fluorescent endpoint genotyping technology of LGC Genomics was developed for 300 SNP loci in castor. The assays were validated in a panel of 13 inbred lines consisting of parents of mapping population and breeding lines with gray mold resistance. The genotyping data quality was high with 100 per cent reproducibility among the replicates. The alternate alleles of the SNP locus and heterozygotes could be clearly discriminated. This genotyping system is highly robust, rapid, cost effective (~ Rs.10 / data point) and will facilitate routine in-house genotyping applications in castor. |
| Funding Type: | External |
| Funding Source: | Biotechnology Industry Research Assistance Council (BIRAC) |
| Subject: | Plant Breeding |
| Keywords: | SNP genotyping KASP assays castor |
| Available Data Format: | PhysicalSamples |
| Language: | English |
| Availability (Extent): | ICAR-IIOR |
| Data Location: | Project Investigator |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/3067 |
| Appears in Collections: | CS-IIOR-Data Inventory |
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