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http://krishi.icar.gov.in/jspui/handle/123456789/3067Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.date.accessioned | 2017-03-16T05:30:29Z | - |
| dc.date.available | 2017-03-16T05:30:29Z | - |
| dc.date.issued | 2016-12-18 | - |
| dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/3067 | - |
| dc.description.abstract | A cost-effective SNP genotyping assay based on ‘Kompetitive Allele Specific PCR genotyping system (KASP), a fluorescent endpoint genotyping technology of LGC Genomics was developed for 300 SNP loci in castor. The assays were validated in a panel of 13 inbred lines consisting of parents of mapping population and breeding lines with gray mold resistance. The genotyping data quality was high with 100 per cent reproducibility among the replicates. The alternate alleles of the SNP locus and heterozygotes could be clearly discriminated. This genotyping system is highly robust, rapid, cost effective (~ Rs.10 / data point) and will facilitate routine in-house genotyping applications in castor. | en_US |
| dc.title | Cost effective SNP genotyping assays for castor | en_US |
| dc.di.divisionUnit | Crop Improvement | en_US |
| dc.di.fundingSource | Biotechnology Industry Research Assistance Council (BIRAC) | en_US |
| dc.di.projectcode | BT/CRS00013/CRS-01/12 | en_US |
| dc.di.funding | External | en_US |
| dc.di.piEmail | senthilvel.senapathy@icar.gov.in | en_US |
| dc.di.piDesignation | Senior Scientist | en_US |
| dc.di.subject | Plant Breeding | en_US |
| dc.di.keywords | SNP genotyping | en_US |
| dc.di.keywords | KASP assays | en_US |
| dc.di.keywords | castor | en_US |
| dc.di.extentLocation | ICAR-IIOR | en_US |
| dc.di.availableDataFormat | PhysicalSamples | en_US |
| dc.di.language | English | en_US |
| dc.di.dataLocation | Project Investigator | en_US |
| dc.contributor.dipiname | Senthilvel Senapathy | en_US |
| dc.di.CurrentLocation | PI | en_US |
| dc.di.PersonEmail | senthilvel.senapathy@icar.gov.in | en_US |
| dc.di.SinceDate | 2016-12-18 | - |
| Appears in Collections: | CS-IIOR-Data Inventory | |
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