Database on spermatozoa transcriptogram of catagorised Frieswal crossbred (Holstein Friesian X Sahiwal) bulls.

Abstract

Bull spermatozoa contain different functional genes and many of them plays important roles in differentstages of spermatogenesis, spermatozoa kinetics, fertilization as well as embryonic development. RNAdeep sequencing is one of the preferred tools for absolute quantification of messenger RNA. The intentionof the current study was to investigate the abundance of spermatozoal transcripts in categorized Frieswal(Holstein-Friesian X Sahiwal) crossbred bull semen through RNA deep sequencing. A total 1546561 and1019308 numbers of reads were identified among good and poor quality bull spermatozoa based on theirconception rate. Post mapping withBos taurusreference genome identified 1,321,236 and 842,022number of transcripts among good and poor quality RNA libraries, respectively. However, a total numberof 3510 and 6759 functional transcripts were identified among good and poor quality bull spermatozoa,respectively. Most of the identified transcripts were related to spermatozoa functions, embryonicdevelopment and other functional aspects of fertilization. Wet laboratory validation of the topfiveselected transcripts (AKAP4, PRM1, ATP2B4, TRIM71 and SLC9B2) illustrated the significant (p<0.01)level of expression in the good quality crossbred bull semen than the poor quality counterparts. Thepresent study with comprehensive profiling of spermatozoal transcripts provides a useful non-invasivetool to understand the causes of as well as an effective way to predict male infertility in crossbred bulls