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Title: | Isolation and characterization of hypoxia inducible heme oxygenase 1 (HMOX1) gene in Labeo rohita |
Other Titles: | Not Available |
Authors: | Rashid, Iliyas Baisvar, Vishwamitra Singh Singh, Mahender Kumar, Ravindra Srivastava, Prachi Kushwaha, Basdeo Pathak, Ajey Kumar |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Bureau of Fish Genetic Resources Amity University, Amity Institute Of Biotechnology, Lucknow Campus, Lucknow 226028, Uttar Pradesh, India |
Published/ Complete Date: | 2020-05 |
Project Code: | Not Available |
Keywords: | HMOX1 Hypoxia Labeo rohita Phylogenetics SSR |
Publisher: | GENOMICS, ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA |
Citation: | Rashid, Iliyas; Baisvar, Vishwamitra Singh; Singh, Mahender; Kumar, Ravindra; Srivastava, Prachi; Kushwaha, Basdeo; Pathak, Ajey Kumar. Isolation and characterization of hypoxia inducible heme oxygenase 1 (HMOX1) gene in Labeo rohita, GENOMICS, 112 (3), 2327-2333. doi:10.1016/j.ygeno.2020.01.004 |
Series/Report no.: | Not Available |
Abstract/Description: | The HMOX1 gene plays role in several biological processes and is also responsive to hypoxia stress. Freshwater carp fish, Labeo rohita, is reported as hypoxia sensitive, but the information of annotated hypoxia genes in public domain is very scanty for this species. Here, an attempt was made to isolate and characterize HMOX1 gene in L. rohita using information from zebrafish. HMOX1 gene was obtained by mapping HMOX1 protein of zebrafish over assembled genome of L. rohita. Aligned region was used for designing primers for HMOX1 amplification. Eight overlapping sets of primers were designed for amplifying similar to 540 bp long successive overlapping fragments. Splicing of overlapping amplicons generated 3715 bp fragment that was confirmed as HMOX1 gene having full coding region with 6 exons between 184 and 2156 bp positions. HMOX1 characterization is an initiative for L. rohita genes annotation to support the characterization of new genes in the important species. |
Description: | Authors are thankful to the Director, ICAR-NBFGR, Lucknow, for providing necessary facilities to carry out this work. Authors are also thankful to the CABin Scheme of ICAR and CABin Division, ICAR-IASRI, New Delhi, for financial assistance and guidance. Authors are highly thankful to the project personnel of the research project entitled `Whole genome sequencing and development of allied genomic resources in two commercially important fish-Labeo rohita and Clarias batrachus' funded by Department of Biotechnology, Government of India, New Delhi, for utilizing the assembled genome sequence of the L. rohita. Authors are also thankful to Department of Biotechnology, Government of India, New Delhi. Authors are indebted to Dr. N S Nagpure, Principal Scientist, ICAR-CIFE, Mumbai, for his support, guidance, suggestions etc. Authors are also grateful to Dr. Satarudra Prakash Singh and Dr. Vineet Awasthi, Amity University Uttar Pradesh, Lucknow campus, for their continued suggestions. |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | CABin Scheme of ICAR CABin Division, ICAR-IASRI, New Delhi Department of Biotechnology, Government of India, New DelhiDepartment of Biotechnology (DBT) India |
Language: | English |
Name of Journal: | Genomics |
NAAS Rating: | 12.21 |
Volume No.: | 112 (3) |
Page Number: | 2327-2333 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1016/j.ygeno.2020.01.004 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/38861 |
Appears in Collections: | FS-NBFGR-Publication |
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