Microbial Synthesis of Phosphorous Nanoparticle from Tri-Calcium Phosphate Using Aspergillus tubingensis TFR-5

Abstract

Innovative approach for the rapid and cost effective biological synthesis of phosphorous nanoparticles was attempted which may be directly used in biomedical, engineering, and agricultural sector. The study demonstrates the synthesis of phosphorous nanoparticle using tri calcium phosphate (Ca3P2O8) as precursor salt by employing Aspergillius tubingensis TFR-5 (NCBI GenBank Accession No. JQ675306). The fungal isolate was identified on the basis of morphological and molecular parameters. Transmission electron microscope (TEM) was used for size and shape study that reveal the spherical structure of monodispersed phosphorous nanoparticle. To ensure the elemental proportion, electron dispersive spectroscopy (EDS) was performed which confirms the 62 atom% of phosphorous. The average size of phosphorous nanoparticle was calculated using dynamic light scattering (DLS) was obtained to be 28.2 nanometer and the polydispersity index (PDI) was 0.435. The crystal structure of phosphorous nanoparticle was confirmed by high resolution transmission electron microscope (HR-TEM) and selective area electron diffraction (SAED) pattern. A possible mechanism for microbial synthesis was also predicted.