Reproductive dysfunction in females of endangered golden mahseer (Tor putitora) in captivity

Abstract

The present study was undertaken to gain insight on the physiological basis underlying the constraints in attaining maturity of endangered golden mahseer (Tor putitora) in captivity. Selected hormone levels and stress biomarkers were analysed in wild and captive reared brooders to address the above objectives. As compared to their captive counterparts, plasma 17β-estradiol was significantly (p < 0.05) higher in wild caught females. A concurrent trend was observed for plasma vitellogenin, aromatase, 17α, 20β-dihydroxy progesterone (17α, 20β DHP), luteinizing hormone (LH) and11-ketotestosterone (11-KT) indicating a weak hormone response in captive females that potentially hindered maturity. To the contrary, the plasma11-KT levels were not significantly different between wild and captive males. Plasma 17α, 20β DHP level was found significantly (p < 0.05) higher in wild caught females compared to females reared in captivity. However, both males of wild and captivity registered significantly higher 17α, 20β DHP than captive females. Plasma 11-KT level was significantly higher in males compared to females. However, the captive females had higher level of 11-KT than captive males. Stress biomarkers viz., cortisol, superoxide dismutase (SOD) and glutathione peroxidase (GPx) were also estimated both in wild and captive brooders. There was no significant difference in plasma cortisol levels of wild and captive reared brooders. However, plasma GPx and SOD activity were significantly higher in captive reared T. putitora as compared to wild brooders counterpart manifesting prevailing oxidative stress in captivity. Overall results showed endocrine and stress differences between wild and captive reared brood fishes during early spawning period which highlighted the endocrine failure of female reproductive maturity in captivity.