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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/42511
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sofia P. Das | en_US |
dc.contributor.author | Amrita Bit | en_US |
dc.contributor.author | Siddhi Patnaik | en_US |
dc.contributor.author | L. Sahoo | en_US |
dc.contributor.author | P. K. Meher | en_US |
dc.contributor.author | P. Jayasankar | en_US |
dc.contributor.author | T. M. Saha | en_US |
dc.contributor.author | A. B. Patel | en_US |
dc.contributor.author | Namrata Patel | en_US |
dc.contributor.author | P. Koringa | en_US |
dc.contributor.author | C. G. Joshi | en_US |
dc.contributor.author | Suyash Agarwal | en_US |
dc.contributor.author | Manmohan Pandey | en_US |
dc.contributor.author | Shreya Srivastava | en_US |
dc.contributor.author | B. Kushwaha | en_US |
dc.contributor.author | Ravindra Kumar | en_US |
dc.contributor.author | N. S. Nagpure | en_US |
dc.contributor.author | M. A. Iquebal | en_US |
dc.contributor.author | Sarika Jaiswal | en_US |
dc.contributor.author | Dinesh Kumar | en_US |
dc.contributor.author | J. K. Jena | en_US |
dc.contributor.author | P. Das | en_US |
dc.date.accessioned | 2020-11-21T05:07:58Z | - |
dc.date.available | 2020-11-21T05:07:58Z | - |
dc.date.issued | 2015-08-11 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/42511 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Labeo rohita, popularly known as rohu, is a widely cultured species in whole Indian subcontinent. In the present study, we used in-silico approach to resolve complete mitochondrial genome of rohu. Low-depth shotgun sequencing using Roche 454 GS FLX (Branford, Connecticut, USA) followed by de novo assembly in CLC Genomics Workbench version 7.0.4 (Aarhus, Denmark) revealed the complete mitogenome of L. rohita to be 16 606 bp long (accession No. KR185963). It comprised of 13 protein-coding genes, 22 tRNAs, 2 rRNAs and 1 putative control region. The gene order and organization are similar to most vertebrates. The mitogenome in the present investigation has 99% similarity with that of previously reported mitogenomes of rohu and this is also evident from the phylogenetic study using maximumlikelihood (ML) tree method. This study was done to determine the feasibility, accuracy and reliability of low-depth sequence data obtained from NGS platform as compared to the Sanger sequencing. Thus, NGS technology has proven to be competent and a rapid in-silico alternative to resolve the complete mitochondrial genome sequence, thereby reducing labors and time | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Taylor and Francis | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | CLC-Bio | en_US |
dc.subject | Labeo rohita | en_US |
dc.subject | mitogenome | en_US |
dc.subject | next-generation sequencing | en_US |
dc.subject | Roche 454 GS FLX | en_US |
dc.title | Low-depth shotgun sequencing resolves complete mitochondrial genome sequence of Labeo rohita | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Article | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Mitochondrial DNA Part A | en_US |
dc.publication.volumeno | 27(5) | en_US |
dc.publication.pagenumber | 3517-3518 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | DOI: 10.3109/19401736.2015.1074197 | en_US |
dc.publication.sourceUrl | http://dx.doi.org/10.3109/19401736.2015.1074197 | en_US |
dc.publication.authorAffiliation | ICAR – Central Institute of Freshwater Aquaculture | en_US |
dc.publication.authorAffiliation | Anand Agricultural University | en_US |
dc.publication.authorAffiliation | ICAR-National Bureau of Fish Genetic Resources | en_US |
dc.publication.authorAffiliation | ICAR-Indian Agricultural Statistics Research Institute | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 7.07 | - |
Appears in Collections: | AEdu-IASRI-Publication |
Files in This Item:
File | Description | Size | Format | |
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Low-depth shotgun sequencing resolves complete mitochondrial genome sequence of Labeo rohita.pdf | 248.79 kB | Adobe PDF | View/Open |
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