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http://krishi.icar.gov.in/jspui/handle/123456789/42931| Title: | Transcriptomic signature of drought response in pearl millet (Pennisetum glaucum) (L.) and development of web-genomic resources |
| Other Titles: | Not Available |
| Authors: | Sarika Jaiswal Tushar J. Antala M. K. Mandavia Meenu Chopra Rahul Singh Jasrotia Rukam S. Tomar Jashminkumar Kheni U. B. Angadi M. A. Iquebal B. A. Golakia Anil Rai Dinesh Kumar |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Junagadh Agricultural University ICAR::Indian Agricultural Statistics Research Institute |
| Published/ Complete Date: | 2018-02-21 |
| Project Code: | Not Available |
| Keywords: | Transcriptomic signature web-genomic resources de novo assembly diferentially expressed genes trait |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Pearl millet, (Pennisetum glaucum L.), an efcient (C4) crop of arid/semi-arid regions is known for hardiness. Crop is valuable for bio-fortifcation combating malnutrition and diabetes, higher caloric value and wider climatic resilience. Limited studies are done in pot-based experiments for drought response at gene-expression level, but feld-based experiment mimicking drought by withdrawal of irrigation is still warranted. We report de novo assembly-based transcriptomic signature of drought response induced by irrigation withdrawal in pearl millet. We found 19983 diferentially expressed genes, 7595 transcription factors, gene regulatory network having 45 hub genes controlling drought response. We report 34652 putative markers (4192 simple sequence repeats, 12111 SNPs and 6249 InDels). Study reveals role of purine and tryptophan metabolism in ABA accumulation mediating abiotic response in which MAPK acts as major intracellular signal sensing drought. Results were validated by qPCR of 13 randomly selected genes. We report the frst web-based genomic resource (http://webtom. cabgrid.res.in/pmdtdb/) which can be used for candidate genes-based SNP discovery programs and trait-based association studies. Looking at climatic change, nutritional and pharmaceutical importance of this crop, present investigation has immense value in understanding drought response in feld condition. This is important in germplasm management and improvement in endeavour of pearl millet productivity. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Scientific Reports |
| NAAS Rating: | 10 |
| Volume No.: | 8 |
| Page Number: | 3382 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | 10.1038/s41598-018-21560-1 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/42931 |
| Appears in Collections: | AEdu-IASRI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Transcriptomic signature of drought response in pearl millet (Pennisetum glaucum (L.) and development of web-genomic resources.pdf | 3.78 MB | Adobe PDF | View/Open |
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