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http://krishi.icar.gov.in/jspui/handle/123456789/43128
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ranjeet R. Kumar | en_US |
dc.contributor.author | Suneha Goswami | en_US |
dc.contributor.author | Mohammad Shamim | en_US |
dc.contributor.author | Kavita Dubey | en_US |
dc.contributor.author | Khushboo Singh | en_US |
dc.contributor.author | Shweta Singh | en_US |
dc.contributor.author | Yugal K. Kala | en_US |
dc.contributor.author | Ravi R. K. Niraj | en_US |
dc.contributor.author | Akshay Sakhrey | en_US |
dc.contributor.author | Gyanendra P. Singh | en_US |
dc.contributor.author | Monendra Grover | en_US |
dc.contributor.author | Bhupinder Singh | en_US |
dc.contributor.author | Gyanendra K. Rai | en_US |
dc.contributor.author | Anil K. Rai | en_US |
dc.contributor.author | Viswanathan Chinnusamy | en_US |
dc.contributor.author | Shelly Praveen | en_US |
dc.date.accessioned | 2020-12-11T08:55:04Z | - |
dc.date.available | 2020-12-11T08:55:04Z | - |
dc.date.issued | 2017-11-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.isbn | Not Available | - |
dc.identifier.issn | 1438-793X | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/43128 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Global warming is a major threat for agriculture and food security, and in many cases the negative impacts are already apparent. Wheat is one of the most important staple food crops and is highly sensitive to the heat stress (HS) during reproductive and grain-filling stages. Here, whole transcriptome analysis of thermotolerant wheat cv. HD2985 was carried out at the post-anthesis stage under control (22 +/- 3 A degrees C) and HS-treated (42 A degrees C, 2 h) conditions using Illumina Hiseq and Roche GS-FLX 454 platforms. We assembled similar to 24 million (control) and similar to 23 million (HS-treated) high-quality trimmed reads using different assemblers with optimal parameters. De novo assembly yielded 52,567 (control) and 59,658 (HS-treated) unigenes. We observed 785 transcripts to be upregulated and 431 transcripts to be downregulated under HS; 78 transcripts showed > 10-fold upregulation such as HSPs, metabolic pathway-related genes, etc. Maximum number of upregulated genes was observed to be associated with processes such as HS-response, protein-folding, oxidation-reduction and photosynthesis. We identified 2008 and 2483 simple sequence repeats (SSRs) markers from control and HS-treated samples; 243 SSRs were observed to be overlying on stress-associated genes. Polymorphic study validated four SSRs to be heat-responsive in nature. Expression analysis of identified differentially expressed transcripts (DETs) showed very high fold increase in the expression of catalytic chaperones (HSP26, HSP17, and Rca) in contrasting wheat cvs. HD2985 and HD2329 under HS. We observed positive correlation between RNA-seq and qRT-PCR expression data. The present study culminated in greater understanding of the heat-response of tolerant genotype and has provided good candidate genes for the marker development and screening of wheat germplasm for thermotolerance. | en_US |
dc.description.sponsorship | Indian Council of Agriculture Research (ICAR) under the National Initiative for Climate Resilient Agriculture (NICRA) project [12/115 TG3079] | en_US |
dc.description.sponsorship | Department of Science and Technology, Government of India (GOI)Department of Science & Technology (India) [SERB/SB/SO/PS/07/2014]; CABin project [TG3064] | en_US |
dc.language.iso | English | en_US |
dc.publisher | FUNCTIONAL & INTEGRATIVE GENOMICS; SPRINGER HEIDELBERG; TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY; HEIDELBERG | en_US |
dc.relation.ispartofseries | Not Available | - |
dc.subject | Abiotic stress | en_US |
dc.subject | DETs | en_US |
dc.subject | DGE | en_US |
dc.subject | De novo assembly | en_US |
dc.subject | Expression profiling | en_US |
dc.subject | Heat stress | en_US |
dc.subject | HSP | en_US |
dc.subject | HSF | en_US |
dc.subject | Next-generation sequencing | en_US |
dc.subject | Novel transcript | en_US |
dc.subject | qRT-PCR | en_US |
dc.subject | Reference set | en_US |
dc.subject | RNA-seq | en_US |
dc.subject | SAGs | en_US |
dc.subject | SAPs | en_US |
dc.subject | Transcriptome | en_US |
dc.subject | Triticum aestivum | en_US |
dc.subject | EXPRESSED SEQUENCE TAGS | en_US |
dc.subject | HIGH-TEMPERATURE STRESS | en_US |
dc.subject | WATER-USE EFFICIENCY | en_US |
dc.subject | TRITICUM-AESTIVUM | en_US |
dc.subject | DROUGHT STRESS | en_US |
dc.subject | RNA-SEQ | en_US |
dc.subject | GENES | en_US |
dc.subject | WILD | en_US |
dc.subject | IMPROVEMENT | en_US |
dc.subject | ANNOTATION | en_US |
dc.title | Exploring the heat-responsive chaperones and microsatellite markers associated with terminal heat stress tolerance in developing wheat | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Article | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Functional and Integrative Genomics | en_US |
dc.publication.volumeno | 17 (6) | en_US |
dc.publication.pagenumber | 621-640 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | http://dx.doi.org/10.1007/s10142-017-0560-1 | en_US |
dc.publication.sourceUrl | https://pubmed.ncbi.nlm.nih.gov/28573536 | en_US |
dc.publication.sourceUrl | Web of Science ID: WOS:000412484200001 | en_US |
dc.publication.authorAffiliation | ICAR::Indian Agricultural Statistics Research Institute | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 9.06 | - |
Appears in Collections: | AEdu-IASRI-Publication |
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