KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/43590
Title: | Isolating Plant Genomic DNA without Liquid Nitrogen |
Other Titles: | Not Available |
Authors: | Sharma R H.R. Mahla T. Mohapatra S.C. Bhargava |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-CAZRI, Jodhpur |
Published/ Complete Date: | 2003-03-01 |
Project Code: | Not Available |
Keywords: | cloning CTAB DNA isolation genomic DNA RAPD restriction |
Publisher: | Not Available |
Citation: | Sharma R, H.R. Mahla, T. Mohapatra and S.C. Bhargava (2003). Isolating Plant Genomic DNA without Liquid Nitrogen. Plant Molecular Biology Reporter 21(1): 43-50. |
Series/Report no.: | Not Available; |
Abstract/Description: | DNA was isolated from leaves of 10 plant species (cuminum cyminum, vigna aconitifolia, pennisetum typhoides, Tecoma stans, Lycium barbarum, Anogeissus acuminate, Tecomella undulata, zizphus mauritiana, Phoenix dactylifera, and Erucasativa ) and a fungus (Fusarium oxysporum ) using the CTAB method. Three fixing solutions (alcohol, alcohol and chloroform , alcohol and EDTA ) were used to produce high molecular weight dna (>40 kb). DNA quality was comparable for the 3 fixing solution offered no advantage over absolute alcohol. Isolated DNA was suitable for RAPD analysis, restriction digestion , abd cloning. This method does not require liquid nitrogen for fixation , grinfing, or storage at- 80 c, making it advantageous over other common protocols. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Plant Molecular Biology |
NAAS Rating: | 9.3 |
Volume No.: | 21(1) |
Page Number: | 43-50 |
Name of the Division/Regional Station: | Div.III (PI&PM) |
Source, DOI or any other URL: | https://link.springer.com/article/10.1007/BF02773395 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/43590 |
Appears in Collections: | NRM-CAZRI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.