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http://krishi.icar.gov.in/jspui/handle/123456789/43606Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Karun Gurjar | en_US |
| dc.contributor.author | Sakshi Rampuria | en_US |
| dc.contributor.author | Uma Joshi | en_US |
| dc.contributor.author | Paramita Palit | en_US |
| dc.contributor.author | K. V. Bhatt | en_US |
| dc.contributor.author | T. Mohapatra | en_US |
| dc.contributor.author | Ramavtar Sharma | en_US |
| dc.date.accessioned | 2020-12-23T11:20:45Z | - |
| dc.date.available | 2020-12-23T11:20:45Z | - |
| dc.date.issued | 2014-06-01 | - |
| dc.identifier.citation | 66. Karun Gurjar & Sakshi Rampuria & Uma Joshi & Paramita Palit & K. V. Bhatt & T. Mohapatra & Ramavtar Sharma. 2014. Identification of Heat-Related ESTs in Moth Bean through Suppression Subtraction Hybridization. Appl Biochem Biotechnol 173 (8), 2116-2128. DOI 10.1007/s12010-014-1011-2 | en_US |
| dc.identifier.issn | Not Available | - |
| dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/43606 | - |
| dc.description | Not Available | en_US |
| dc.description.abstract | Abstract Moth bean (Vigna aconitifolia (Jacq.) Marechal), an important grain-legume crop grown in hot desert regions of Thar, under scorching sun rays, was investigated for heat tolerance at molecular level. In the present study, we constructed a forward suppression subtractive hybridization (SSH) cDNA library of heat tolerant genotype RMO-40 to identify genes expressing under delayed response to elevated temperature. Heat induction was carried out by exposing 14-day-old seedlings to elevated temperature of 42 °C for 30 min. A total of 125 unigenes (33 contigs and 92 singletons) were derived by cluster assembly and sequence alignment of 200 ESTs; out of 125 unigenes, 21 (16 %) were found to be novel to moth bean. Gene ontology functional classification terms were retrieved for 98 (78.4 %) unigenes of | en_US |
| dc.description.sponsorship | Not Available | en_US |
| dc.language.iso | English | en_US |
| dc.publisher | Not Available | en_US |
| dc.relation.ispartofseries | Not Available; | - |
| dc.subject | Moth bean | en_US |
| dc.subject | SSH cDNA library | en_US |
| dc.subject | Sequence analysis | en_US |
| dc.subject | HSPs | en_US |
| dc.subject | Semi-qRT-PCR | en_US |
| dc.subject | assay | en_US |
| dc.title | Identification of Heat-Related ESTs in Moth Bean Through Suppression Subtraction Hybridization | en_US |
| dc.title.alternative | Not Available | en_US |
| dc.type | Research Paper | en_US |
| dc.publication.projectcode | Not Available | en_US |
| dc.publication.journalname | Applied Biochemistry and Biotechnology | en_US |
| dc.publication.volumeno | 173 (8) | en_US |
| dc.publication.pagenumber | 2116-2128 | en_US |
| dc.publication.divisionUnit | Div.III (PI&PM) | en_US |
| dc.publication.sourceUrl | 10.1007/s12010-014-1011-2 | en_US |
| dc.publication.authorAffiliation | ICAR::Central Arid Zone Research Institute | en_US |
| dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
| dc.publication.naasrating | 8.28 | en_US |
| Appears in Collections: | NRM-CAZRI-Publication | |
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