KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/4374
Title: | Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol |
Other Titles: | Not Available |
Authors: | Bhauso, T.D. Radhakrishnan, T. Kumar, A. Mishra, G.P. Dobaria, J.R. Patel, K. Rajam, M.V. |
Published/ Complete Date: | 2014-10-19 |
Project Code: | IXX10033 |
Keywords: | mtld drought Drought Tolerance Peanut Groudnut mannitol |
Publisher: | Hindwai Publisher |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | In the changing global environmental scenarios, water scarcity and recurrent drought impose huge reductions to the peanut (Arachis hypogaea L.) crop yield. In plants, osmotic adjustments associated with efficient free radical scavenging ability during abiotic stress are important components of stress tolerance mechanisms. Mannitol, a compatible solute, is known to scavenge hydroxyl radicals generated during various abiotic stresses, thereby conferring tolerance to water-deficit stress in many plant species. However, peanut plant is not known to synthesize mannitol. Therefore, bacterial mtlD gene coding for mannitol 1-phosphate dehydrogenase under the control of constitutive promoter CaMV35S was introduced and overexpressed in the peanut cv. GG 20 using Agrobacterium tumefaciens-mediated transformation. A total of eight independent transgenic events were confirmed at molecular level by PCR, Southern blotting, and RT-PCR. Transgenic lines had increased amount of mannitol and exhibited enhanced tolerance in response to water-deficit stress. Improved performance of the mtlD transgenics was indicated by excised-leaf water loss assay and relative water content under water-deficit stress. Better performance of transgenics was due to the ability of the plants to synthesize mannitol. However, regulation of mtlD gene expression in transgenic plants remains to be elucidated. |
Description: | Not Available |
ISSN: | 2356-6140 1537-744X |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | The Scientific World Journal |
NAAS Rating: | Not Available |
Volume No.: | 2014 |
Page Number: | 1-10 |
Name of the Division/Regional Station: | Crop Improvement |
Source, DOI or any other URL: | 10.1155/2014/125967 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/4374 |
Appears in Collections: | CS-DGR-Publication |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
ICAR_DGR_Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol.pdf | 3.34 MB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.