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http://krishi.icar.gov.in/jspui/handle/123456789/4388| Title: | Influence of BAP on in vitro regeneration of shoots from immature leaves of groundnut (Arachis hypogaea L.) |
| Other Titles: | Not Available |
| Authors: | Yadav, R. Radhakrishnan, T. Dobaria, J.R. Kumar, A. |
| Published/ Complete Date: | 2013-03-01 |
| Project Code: | Not Available |
| Keywords: | Arachis hypogaea BAP Immature leaves Regeneration |
| Publisher: | Journal of Plant Development Sciences |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Cytokinins are used in in vitro protocols singly or in combinations with auxins to induce cell proliferation and promote shoot regeneration. We report a protocol for efficient regeneration of immature leaf explants from groundnut (Arachis hypogaea L.) var. Kadiri-6 and K-134 using a combination of NAA and BAP. A maximum of 90% regeneration with more than 7 shoots per explant was obtained from explants cultured on MS medium with 4 mg/L BAP and 1 mg/L NAA with subsequent substitutions of NAA with AgNO3 for shoot induction and AgNO3 by GA3 for elongation of the shoots. The levels of BAP in the culture medium significantly influenced the frequency of regeneration. This protocol of indirect regeneration from the immature leaves may be used in genetic transformation protocols of groundnut with higher efficiency of recovery of plantlets. |
| Description: | Not Available |
| ISSN: | 0974-6382 |
| Type(s) of content: | Research Paper |
| Sponsors: | DBT |
| Language: | English |
| Name of Journal: | Journal of Plant Development Sciences |
| NAAS Rating: | 4.13 |
| Volume No.: | 5 |
| Page Number: | 1-4 |
| Name of the Division/Regional Station: | Crop Improvement |
| Source, DOI or any other URL: | http://jpds.co.in/download/new/Vol.%205(1).pdf |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/4388 |
| Appears in Collections: | CS-DGR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ICAR_DGR_Influence of BAP on in vitro regeneration of shoots from immature leaves of groundnut (Arachis hypogaea L.)..pdf | 228.2 kB | Adobe PDF | View/Open |
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