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http://krishi.icar.gov.in/jspui/handle/123456789/44214Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Kumar S | en_US |
| dc.contributor.author | Singh G | en_US |
| dc.contributor.author | Jat RS | en_US |
| dc.contributor.author | Saxena SN | en_US |
| dc.contributor.author | Sharma R | en_US |
| dc.date.accessioned | 2020-12-29T10:06:30Z | - |
| dc.date.available | 2020-12-29T10:06:30Z | - |
| dc.date.issued | 2010-12-01 | - |
| dc.identifier.citation | Kumar S, Singh G, Jat RS, Saxena SN and Sharma R. 2010. Transformation of mothbean (Vigna aconotifolia) cotyledonary nodes through vacuum infiltration of Agrobacterium tumefaciens. Green Farming Vol. 1(6) : 551-554. | en_US |
| dc.identifier.issn | Not Available | - |
| dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/44214 | - |
| dc.description | Not Available | en_US |
| dc.description.abstract | The present investigation generates a rapid and convenient stable expression protocol based on vacuum infiltration of Agrobacterium tumefaciens cells into moth bean cotyledonary nodes. Vacuum infiltrated tissues showed transient GUS expression than did non infiltrated tissues. The leaflets arising from the transformed shoots on kanamycin supplemented media were found GUS positive during histochemical assay. We believe this to be the first report of this technique for putative gene expression in mothbean cotyledonary nodes. Mothbean cotyledonary nodes are one of the most regenerative tissues in legumes. | en_US |
| dc.description.sponsorship | Not Available | en_US |
| dc.language.iso | English | en_US |
| dc.publisher | Kumar S, Singh G, Jat RS, Saxena SN and Sharma R. 2010 | en_US |
| dc.relation.ispartofseries | Not Available; | - |
| dc.subject | Agrobacterium tumefaciens | en_US |
| dc.subject | cotyledonary nodes | en_US |
| dc.subject | mothbean | en_US |
| dc.subject | vacuum infiltration | en_US |
| dc.subject | Vigna aconitifolia | en_US |
| dc.title | Transformation of mothbean (Vigna aconotifolia) cotyledonary nodes through vacuum infiltration of Agrobacterium tumefaciens | en_US |
| dc.title.alternative | Not Available | en_US |
| dc.type | Research Paper | en_US |
| dc.publication.projectcode | Not Available | en_US |
| dc.publication.journalname | Green Farming | en_US |
| dc.publication.volumeno | 1(6) | en_US |
| dc.publication.pagenumber | 551-554 | en_US |
| dc.publication.divisionUnit | Div.III (PI&PM) | en_US |
| dc.publication.sourceUrl | ISSN 0974-0775 | en_US |
| dc.publication.sourceUrl | https://www.greenfarming.in/?articles=transformation-of-mothbean-vigna-aconitifolia-cotyledonary-nodes-through-vacuum-infiltration-of-agrobacterium-tumefaciens | en_US |
| dc.publication.authorAffiliation | ICAR::Central Arid Zone Research Institute | en_US |
| dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
| dc.publication.naasrating | 3.85 | en_US |
| Appears in Collections: | NRM-CAZRI-Publication | |
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