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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/45804
Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Snatashree Mohanty | en_US |
dc.contributor.author | Makesh, M. | en_US |
dc.contributor.author | Rajendran , K.V. | en_US |
dc.contributor.author | Suresh Babu | en_US |
dc.contributor.author | Deepika Anand | en_US |
dc.contributor.author | Saurav Kumar | en_US |
dc.contributor.author | Abhay Kumar | en_US |
dc.contributor.author | Raju Baitha | en_US |
dc.contributor.author | Kamal Sarma | en_US |
dc.date.accessioned | 2021-03-04T06:12:34Z | - |
dc.date.available | 2021-03-04T06:12:34Z | - |
dc.date.issued | 2020-04 | - |
dc.identifier.citation | Snatashree Mohanty, Makesh, M., Rajendran , K.V., Suresh Babu, Deepika Anand, Saurav Kumar, Abhay Kumar, Raju Baitha and Kamal Sarma (2020) Production and characterisation of monoclonal antibodies against immunoglobulins of Cirrhinusmrigala (Hamilton 1822) Indian J. Fish., 67(2): 55-61. | en_US |
dc.identifier.issn | 0970-6011 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/45804 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Serum immunoglobulins (Ig) of mrigal Cirrhinus mrigala (Hamilton 1822) immunised with bovine serum albumin (BSA), were purifed by affnity chromatography using BSA-CL agarose column. The purifed mrigal Ig (m-Ig) was characterised under reducing condition by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) which revealed two bands of 85 and 26 kDa corresponding to heavy and light chain, respectively. Following fusion of splenocytes from Balb/c mice immunised with purifed m-Ig with myeloma cells, three hybridomas showing reactivity with m-Ig were cloned by limiting dilution. The monoclonal antibodies (MAbs) generated by these clones were designated as 3B2-E12, 3B2-F9 and 4C3-B2 and characterised by western blotting and isotyping. Western blot analysis of the supernatant from the three clones with purifed m-Ig indicated that, all the three MAbs were specifc to heavy chain. Isotyping revealed that 3B2-E12 MAb was of IgG1 isotype whereas the other two MAbs were of IgG2a isotype. Cross reactivity of anti-mrigal Ig MAb (3B2-E12) was observed with serum Ig of Catla catla and Labeo rohita indicating semi-conserved nature of Ig in Indian major carps. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Indian Council of Agricultural Research | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Cirrhinus mrigala | en_US |
dc.subject | ELISA | en_US |
dc.subject | Immunoglobulin | en_US |
dc.subject | Monoclonal Antibodies | en_US |
dc.subject | Western blotting | en_US |
dc.title | Production and characterisation of monoclonal antibodies against immunoglobulins of Cirrhinus mrigala (Hamilton 1822) | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Indian Journal of Fisheires | en_US |
dc.publication.volumeno | 67(2) | en_US |
dc.publication.pagenumber | 55-61 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | 10.21077/ijf.2019.67.2.84594-08 | en_US |
dc.publication.authorAffiliation | ICAR::Central Institute of Freshwater Aquaculture | en_US |
dc.publication.authorAffiliation | ICAR::Central Institute of Brackishwater Aquaculture | en_US |
dc.publication.authorAffiliation | ICAR::Central Institute of Fisheries Education | en_US |
dc.publication.authorAffiliation | ICAR::Central Marine Fisheries Research Institute | en_US |
dc.publication.authorAffiliation | ICAR::Central Institute of Fisheries Technology | en_US |
dc.publication.authorAffiliation | ICAR::Central Inland Fisheries Research Institute | en_US |
dc.publication.authorAffiliation | ICAR-Research Complex for Eastern Region, Patna - 800 014, Bihar, India | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 6.29 | en_US |
Appears in Collections: | FS-CIFT-Publication |
Files in This Item:
File | Description | Size | Format | |
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Production and characterisation of monoclonal antibodies.pdf | 959.73 kB | Adobe PDF | View/Open |
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