KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/45804| Title: | Production and characterisation of monoclonal antibodies against immunoglobulins of Cirrhinus mrigala (Hamilton 1822) |
| Other Titles: | Not Available |
| Authors: | Snatashree Mohanty Makesh, M. Rajendran , K.V. Suresh Babu Deepika Anand Saurav Kumar Abhay Kumar Raju Baitha Kamal Sarma |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Institute of Freshwater Aquaculture ICAR::Central Institute of Brackishwater Aquaculture ICAR::Central Institute of Fisheries Education ICAR::Central Marine Fisheries Research Institute ICAR::Central Institute of Fisheries Technology ICAR::Central Inland Fisheries Research Institute ICAR-Research Complex for Eastern Region, Patna - 800 014, Bihar, India |
| Published/ Complete Date: | 2020-04 |
| Project Code: | Not Available |
| Keywords: | Cirrhinus mrigala ELISA Immunoglobulin Monoclonal Antibodies Western blotting |
| Publisher: | Indian Council of Agricultural Research |
| Citation: | Snatashree Mohanty, Makesh, M., Rajendran , K.V., Suresh Babu, Deepika Anand, Saurav Kumar, Abhay Kumar, Raju Baitha and Kamal Sarma (2020) Production and characterisation of monoclonal antibodies against immunoglobulins of Cirrhinusmrigala (Hamilton 1822) Indian J. Fish., 67(2): 55-61. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Serum immunoglobulins (Ig) of mrigal Cirrhinus mrigala (Hamilton 1822) immunised with bovine serum albumin (BSA), were purifed by affnity chromatography using BSA-CL agarose column. The purifed mrigal Ig (m-Ig) was characterised under reducing condition by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) which revealed two bands of 85 and 26 kDa corresponding to heavy and light chain, respectively. Following fusion of splenocytes from Balb/c mice immunised with purifed m-Ig with myeloma cells, three hybridomas showing reactivity with m-Ig were cloned by limiting dilution. The monoclonal antibodies (MAbs) generated by these clones were designated as 3B2-E12, 3B2-F9 and 4C3-B2 and characterised by western blotting and isotyping. Western blot analysis of the supernatant from the three clones with purifed m-Ig indicated that, all the three MAbs were specifc to heavy chain. Isotyping revealed that 3B2-E12 MAb was of IgG1 isotype whereas the other two MAbs were of IgG2a isotype. Cross reactivity of anti-mrigal Ig MAb (3B2-E12) was observed with serum Ig of Catla catla and Labeo rohita indicating semi-conserved nature of Ig in Indian major carps. |
| Description: | Not Available |
| ISSN: | 0970-6011 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Fisheires |
| NAAS Rating: | 6.29 |
| Volume No.: | 67(2) |
| Page Number: | 55-61 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | 10.21077/ijf.2019.67.2.84594-08 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/45804 |
| Appears in Collections: | FS-CIFT-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Production and characterisation of monoclonal antibodies.pdf | 959.73 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.