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http://krishi.icar.gov.in/jspui/handle/123456789/46429
Title: | Development and validation of high throughput real-time polymerase chain reaction assay for quantitative detection of chicken infectious anemia virus |
Other Titles: | Not Available |
Authors: | T. R. Kannaki E. Priyanka Madhuri Subbiah Santosh Haunshi |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR:Directorate of Poultry Research National Institute of Animal Biotechnology |
Published/ Complete Date: | 2021-04-01 |
Project Code: | Not Available |
Keywords: | Chicken infectious anemia ∙ Real-time PCR |
Publisher: | Springer |
Series/Report no.: | Not Available; |
Abstract/Description: | A real time polymerase chain reaction (real-time PCR) assay was developed to detect and quantify the chicken infectious anemia virus (CIAV). The two sets of primers specific to VP1 region of CIAV were designed and their sensitivity and efficacy were studied. Both the primers designed in this study were highly sensitive and were able to detect upto 0.01 fg/µl or 82 x 102 copy number of plasmid DNA. The efficiency of the real time PCR was 100.9 %. The results have also shown that the present qPCR assay is 100 times more sensitive than regular qualitative PCR. Both primer sets were validated using 28 field poultry samples and showed good results. The optimized real-time quantitative PCR will be useful in quick detection of field outbreaks, sub-clinical infection in poultry flocks, virus pathogenesis studies and for detecting vaccine contamination. |
Description: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Virus Disease |
Journal Type: | Research Journal |
NAAS Rating: | 5.95 |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1007/s13337-020-00648-1 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/46429 |
Appears in Collections: | AS-DPR-Publication |
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