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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/46429
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | T. R. Kannaki | en_US |
dc.contributor.author | E. Priyanka | en_US |
dc.contributor.author | Madhuri Subbiah | en_US |
dc.contributor.author | Santosh Haunshi | en_US |
dc.date.accessioned | 2021-04-12T09:33:45Z | - |
dc.date.available | 2021-04-12T09:33:45Z | - |
dc.date.issued | 2021-04-01 | - |
dc.identifier.other | 10.1007/s13337-020-00648-1 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/46429 | - |
dc.description | Not Available | en_US |
dc.description.abstract | A real time polymerase chain reaction (real-time PCR) assay was developed to detect and quantify the chicken infectious anemia virus (CIAV). The two sets of primers specific to VP1 region of CIAV were designed and their sensitivity and efficacy were studied. Both the primers designed in this study were highly sensitive and were able to detect upto 0.01 fg/µl or 82 x 102 copy number of plasmid DNA. The efficiency of the real time PCR was 100.9 %. The results have also shown that the present qPCR assay is 100 times more sensitive than regular qualitative PCR. Both primer sets were validated using 28 field poultry samples and showed good results. The optimized real-time quantitative PCR will be useful in quick detection of field outbreaks, sub-clinical infection in poultry flocks, virus pathogenesis studies and for detecting vaccine contamination. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Springer | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Chicken infectious anemia ∙ Real-time PCR | en_US |
dc.title | Development and validation of high throughput real-time polymerase chain reaction assay for quantitative detection of chicken infectious anemia virus | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Virus Disease | en_US |
dc.publication.volumeno | Not Available | en_US |
dc.publication.pagenumber | Not Available | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://doi.org/10.1007/s13337-020-00648-1 | en_US |
dc.publication.authorAffiliation | ICAR:Directorate of Poultry Research | en_US |
dc.publication.authorAffiliation | National Institute of Animal Biotechnology | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | Research Journal | en_US |
dc.publication.naasrating | 5.95 | en_US |
Appears in Collections: | AS-DPR-Publication |
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