KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/47293
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shashi Rawat | - |
dc.date.accessioned | 2021-06-14T10:49:54Z | - |
dc.date.available | 2021-06-14T10:49:54Z | - |
dc.date.issued | 2020-1-1 | - |
dc.identifier.citation | Jagesh Kumar Tiwari, Tanuja Buckseth, Rasna Zinta, Aastha Saraswati, Rajesh Kumar Singh, Shashi Rawat, Vijay Kumar Dua & Swarup Kumar Chakrabarti | en_US |
dc.identifier.isbn | Not Available | en_US |
dc.identifier.issn | 2045-2322 | en_US |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/47293 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Potato crop requires high dose of nitrogen (N) to produce high tuber yield. Excessive application of N causes environmental pollution and increases cost of production. Hence, knowledge about genes and regulatory elements is essential to strengthen research on N metabolism in this crop. In this study, we analysed transcriptomes (RNA-seq) in potato tissues (shoot, root and stolon) collected from plants grown in aeroponic culture under controlled conditions with varied N supplies i.e. low N (0.2 milli molar N) and high N (4 milli molar N). High quality data ranging between 3.25 to 4.93 Gb per sample were generated using Illumina NextSeq500 that resulted in 83.60?86.50% mapping of the reads to the reference potato genome. Differentially expressed genes (DEGs) were observed in the tissues based on statistically significance (p ? 0.05) and up-regulation with ? 2 log2 fold change (FC) and down-regulation with ? ?2 log2 FC values. In shoots, of total 19730 DEGs, 761 up-regulated and 280 down-regulated significant DEGs were identified. Of total 20736 DEGs in roots, 572 (up-regulated) and 292 (down-regulated) were significant DEGs. In stolons, of total 21494 DEG, 688 and 230 DEGs were significantly up-regulated and down-regulated, respectively. Venn diagram analysis showed tissue specific and common genes. The DEGs were functionally assigned with the GO terms, in which molecular function domain was predominant in all the tissues. Further, DEGs were classified into 24 KEGG pathways, in which 5385, 5572 and 5594 DEGs were annotated in shoots, roots and stolons, respectively. The RT-qPCR analysis validated gene expression of RNA-seq data for selected genes. We identified a few potential DEGs responsive to N deficiency in potato such as lutaredoxin, Myb-like DNA-binding protein, WRKY transcription factor 16 and FLOWERING LOCUS T in shoots; high-affinity nitrate transporter, protein phosphatase-2c, glutaredoxin family protein, malate synthase, CLE7, 2-oxoglutarate-dependent dioxygenase and transcription factor in roots; and glucose-6-phosphate/ phosphate translocator 2, BTB/POZ domain-containing protein, F-box family protein and aquaporin TIP1;3 in stolons, and many genes of unknown function. Our study highlights that these potential genes play very crucial roles in N stress tolerance, which could be useful in augmenting research on N metabolism in potato. | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.subject | Potato,nitrogen stress | en_US |
dc.title | Transcriptome analysis of potato shoots, roots and stolons under nitrogen stress | en_US |
dc.type | Research Paper | en_US |
dc.publication.journalname | Scientific Reports | en_US |
dc.publication.submitter | From ARMS | - |
Appears in Collections: | AEng-CIAE-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.