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http://krishi.icar.gov.in/jspui/handle/123456789/48569| Title: | A Simple and Quick DNA Extraction Procedure for Rapid Diagnosis of Sex of Chicken and Chicken Embryos |
| Other Titles: | Not Available |
| Authors: | Santosh Haunshi Pattanayak, A. Bandyopadhaya, S. Bujarbaruah, K. M. Saxena, S. C. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Research Complex for NEH Region ICAR::Directorate of Poultry Research |
| Published/ Complete Date: | 2008-01-01 |
| Project Code: | Not Available |
| Keywords: | Alkaline, chicken, DNA extraction, feather, sexing |
| Publisher: | Japan Poultry Science Association |
| Citation: | Haunshi S., Pattanayak, A., Bandyopadhaya, S., Bujarbaruah, K.M. Saxena, S.C. 2008. A Simple and Quick DNA Extraction Procedure for Rapid Diagnosis of Sex of Chicken and Chicken Embryos. The Journal of Poultry Science, 45(1):75-81. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The objective of this study was to develop a simple and quick DNA extraction procedure for rapid diagnosis of sex of chicken and its embryos using multiplex polymerase chain reaction (PCR). Using alkaline method of DNA extraction from whole blood and feather bulb of adults, and tissue samples from embryos, the present study demonstrated that identification of sex by multiplex PCR protocol is simplest, safer, faster and inexpensive. Multiplex PCR was used to amplify W chromosome specific 481-bp fragment in female and 256bp fragment of 18S ribosomal gene in both male and female chicken. DNA samples were prepared by conventional phenol-chloroform-iso-amyl alcohol (PCI) method, modified PCI method, wizard genomic DNA purification kit and a simple alkaline method from blood samples and feather bulbs of adults and tissue samples of embryos. The protocol successfully identified sex of embryos and White Leghorn (Exotic), indigenous and Vanaraja (Hybrid of exotic and indigenous) varieties of chicken. Sequence comparison of W chromosome specific PCR products amplified from these three varieties showed no difference among them. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | SERC |
| Language: | English |
| Name of Journal: | Journal of Poultry Science |
| Journal Type: | Research Journal |
| NAAS Rating: | 6.88 |
| Impact Factor: | 1.425 |
| Volume No.: | 45(1) |
| Page Number: | 75-81 |
| Name of the Division/Regional Station: | Poultry Science |
| Source, DOI or any other URL: | https://doi.org/10.2141/jpsa.45.75 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/48569 |
| Appears in Collections: | AS-DPR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| J Poult Sci 2008.pdf | 313.57 kB | Adobe PDF | View/Open |
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