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http://krishi.icar.gov.in/jspui/handle/123456789/4940
Title: | Monoclonal antibodies to snakehead, Channa striata immunoglobulins: Detection and quantification of immunoglobulin-positive cells in blood and lymphoid organs |
Other Titles: | Not Available |
Authors: | Neeraj Sood Dharmendra K. Chaudhary Gaurav Rathore Akhilesh Singh W.S. Lakra |
Published/ Complete Date: | 2011 |
Project Code: | BT/PR9275/AAQ/03/335/2007 |
Keywords: | Channa striata ELISA Flow cytometry Immunoglobulin Immunoperoxidase test Monoclonal antibody |
Publisher: | ELSEVIER |
Citation: | Sood N, Chaudhary DK, Rathore G, Singh A, Lakra WS. Monoclonal antibodies to snakehead, Channa striata immunoglobulins: detection and quantification of immunoglobulin-positive cells in blood and lymphoid organs. Fish Shellfish Immunol. 2011 Feb;30(2):569-75. doi: 10.1016/j.fsi.2010.12.004. Epub 2010 Dec 15. PubMed PMID: 21167285. |
Series/Report no.: | Not Available; |
Abstract/Description: | Snakehead Channa striata is an important freshwater food fish in many Southeast Asian countries. Three monoclonal antibodies (C9, C10 and D10) were developed against purified serum immunoglobulins of Channa striata (Cs-Ig) and characterized. C9 and D10 MAbs were specific to heavy chain, while C10 MAb detected only unreduced Cs-Ig in western blotting. In competitive ELISA, C9 and C10 MAbs were specific to C. striata Ig and showed no cross reactivity with serum Ig of other fish species i.e. Channa punctatus, Channa marulius, Clarias batrachus and Labeo rohita. D10 MAb showed reactivity to serum Ig of C. striata and C. marulius. In FACS analysis of gated lymphocytes, the percentage of Igþ cells detected by C9 MAb was 18.2%, 27.7% and 10.3% in blood, spleen and kidney, respectively (n ¼ 3, body weight 500e600 g). However, only a few cells (0.5%) were found to be Igþ in thymus (n ¼ 5). C9 MAb was also successfully employed to demonstrate Igþ cells in blood smears and formalin fixed sections of spleen and kidney. These findings suggest that the spleen plays an important role in humoral immunity as compared to head kidney. Further, these MAbs can be useful immunological tool in monitoring health status of cultured C. striata. |
Description: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Department of Biotechnology, Ministry of Science and Technology, Government of India . |
Language: | English |
Name of Journal: | Fish and Shellfish Immunology |
NAAS Rating: | 6 |
Volume No.: | 30 |
Page Number: | 569-575 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | 10.1016/j.fsi.2010.12.004 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/4940 |
Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
File | Description | Size | Format | |
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1-s2.0-S1050464810003761-main.pdf | 1.16 MB | Adobe PDF | View/Open |
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