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http://krishi.icar.gov.in/jspui/handle/123456789/4940
Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Neeraj Sood | en_US |
dc.contributor.author | Dharmendra K. Chaudhary | en_US |
dc.contributor.author | Gaurav Rathore | en_US |
dc.contributor.author | Akhilesh Singh | en_US |
dc.contributor.author | W.S. Lakra | en_US |
dc.date.accessioned | 2017-07-24T06:14:38Z | - |
dc.date.available | 2017-07-24T06:14:38Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Sood N, Chaudhary DK, Rathore G, Singh A, Lakra WS. Monoclonal antibodies to snakehead, Channa striata immunoglobulins: detection and quantification of immunoglobulin-positive cells in blood and lymphoid organs. Fish Shellfish Immunol. 2011 Feb;30(2):569-75. doi: 10.1016/j.fsi.2010.12.004. Epub 2010 Dec 15. PubMed PMID: 21167285. | en_US |
dc.identifier.other | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/4940 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Snakehead Channa striata is an important freshwater food fish in many Southeast Asian countries. Three monoclonal antibodies (C9, C10 and D10) were developed against purified serum immunoglobulins of Channa striata (Cs-Ig) and characterized. C9 and D10 MAbs were specific to heavy chain, while C10 MAb detected only unreduced Cs-Ig in western blotting. In competitive ELISA, C9 and C10 MAbs were specific to C. striata Ig and showed no cross reactivity with serum Ig of other fish species i.e. Channa punctatus, Channa marulius, Clarias batrachus and Labeo rohita. D10 MAb showed reactivity to serum Ig of C. striata and C. marulius. In FACS analysis of gated lymphocytes, the percentage of Igþ cells detected by C9 MAb was 18.2%, 27.7% and 10.3% in blood, spleen and kidney, respectively (n ¼ 3, body weight 500e600 g). However, only a few cells (0.5%) were found to be Igþ in thymus (n ¼ 5). C9 MAb was also successfully employed to demonstrate Igþ cells in blood smears and formalin fixed sections of spleen and kidney. These findings suggest that the spleen plays an important role in humoral immunity as compared to head kidney. Further, these MAbs can be useful immunological tool in monitoring health status of cultured C. striata. | en_US |
dc.description.sponsorship | Department of Biotechnology, Ministry of Science and Technology, Government of India . | en_US |
dc.language.iso | English | en_US |
dc.publisher | ELSEVIER | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Channa striata | en_US |
dc.subject | ELISA | en_US |
dc.subject | Flow cytometry | en_US |
dc.subject | Immunoglobulin | en_US |
dc.subject | Immunoperoxidase test | en_US |
dc.subject | Monoclonal antibody | en_US |
dc.title | Monoclonal antibodies to snakehead, Channa striata immunoglobulins: Detection and quantification of immunoglobulin-positive cells in blood and lymphoid organs | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | BT/PR9275/AAQ/03/335/2007 | en_US |
dc.publication.journalname | Fish and Shellfish Immunology | en_US |
dc.publication.volumeno | 30 | en_US |
dc.publication.pagenumber | 569-575 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | 10.1016/j.fsi.2010.12.004 | en_US |
dc.publication.naasrating | 6 | en_US |
Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
File | Description | Size | Format | |
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1-s2.0-S1050464810003761-main.pdf | 1.16 MB | Adobe PDF | View/Open |
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