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http://krishi.icar.gov.in/jspui/handle/123456789/49433
Title: | In vitro propagation of spine gourd (Momordica dioica Roxb.) and assessment of genetic fidelity of micropropagated plants using RAPD analysis |
Other Titles: | Not Available |
Authors: | Rai, Govind Kumar Singh, Major Rai, Neha Prakash Bhardwaj, D.R. Kumar Sanjeev |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-Indian Institute of Vegetable Research, Varanasi (U.P.) |
Published/ Complete Date: | 2012-04-20 |
Project Code: | Not Available |
Keywords: | Axillary shoot proliferation; Dioecious; Genetic fidelity; Micropropagation; Momordica dioica; Nodal explants. |
Publisher: | Physiology and Molecular Biology of Plants is published by Springer India |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | An efficient protocol for rapid in vitro clonal propagation of spine gourd (Momordica dioica Roxb.) genotype RSR/DR15 (female) and DR/NKB-28 (male) was developed through enhanced axillary shoot proliferation from nodal segments. Maximum shoot proliferation of 6.2 shoots per explant with 100 % shoot regeneration frequency was obtained from the female genotype on Murashige and Skoog's (1962) medium supplemented with 0.9 μM N6-benzyladenine (BA) and 200 mg l(-1) casein hydrolysate (CH). While from the male genotype the optimum shoot regeneration frequency (86.6 %) and 6.4 shoots per explant was obtained on MS medium supplemented with 2.2 μM BA. CH induced vigorous shoots, promoted callus formation, and proved inhibitory for shoot differentiation and shoot length, especially in explants from male genotype. Rooting was optimum on half-strength MS medium (male 92.8 %, female 74.6 %) containing 4.9 μM indole-3-butyric acid (IBA). Plantlets were transferred to plastic cups containing a mixture of cocopit and perlite (1:1 ratio) and then to soil after 2-3 weeks. 84 % female and 81 % male regenerated plantlets survived and grew vigorously in the field. Genetic stability of the regenerated plants was assessed using random amplified polymorphic DNA (RAPD). The amplification products were monomorphic in the in vitro propagated plants and similar to those of mother plant. No polymorphism was detected revealing the genetic integrity of in vitro propagated plants. This micropropagation procedure could be useful for raising genetically uniform planting material of known sex for commercial cultivation or build-up of plant material of a specific sex-type. |
Description: | Not Available |
ISSN: | 09740430, 09715894 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Physiology and Molecular Biology of Plants |
Journal Type: | Research Journal |
NAAS Rating: | 8.01 |
Volume No.: | 18(3) |
Page Number: | 273-280 |
Name of the Division/Regional Station: | Division of Crop Improvement |
Source, DOI or any other URL: | DOI: 10.1007/s12298-012-0109-7 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/49433 |
Appears in Collections: | HS-IIVR-Publication |
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