KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/5034
Title: | PCR Cloning and Partial Sequencing of rtxA Gene of non-O1/non-O139 Vibrio cholerae Isolated from Gold Fish Carassius auratus in India |
Other Titles: | Not Available |
Authors: | T. RAJA SWAMINATHAN NEERAJ SOOD GAURAV RATHORE REHANA ABIDI W. S. LAKRA |
Published/ Complete Date: | 2009 |
Project Code: | Not Available |
Keywords: | cholerae Isolated Gold Fish Carassius auratus PCR Cloning Partial Sequencing |
Publisher: | Asian Fisheries Society |
Citation: | Swaminathan, T.R., Sood, N., Rathore, G., Abidi, R. and Lakra, W.S., 2009. PCR cloning and partial sequencing of rtxA gene of non-O1/non-O139 Vibrio cholerae isolated from gold fish Carassius auratus in India. Asian Fisheries Science, 22(2), pp.707-712. |
Series/Report no.: | Not Available; |
Abstract/Description: | A total of 16 non-O1/ non-O139 Vibrio cholerae isolates were obtained from ornamental fish collected from aquarium traders located in four metro cities of India. All the isolates were confirmed by amplifying the 300 bp fragment in the 16S-23S rRNA intergenic spacer regions. Amplification of 431 bp fragment of gene, which resulted in detectable levels of PCR product,was achieved with a minimum of 8 CFU/ml of V. cholerae. The detection limit for rtxA gene by PCR amplification of genomic DNA was 20 picogram. A 431 bp fragment of the rtxA gene of V. cholerae was cloned and sequenced (NCBI accession number EU714289). Nucleotide sequence analysis data of the rtxA gene showed that about 96% identical to those of V. cholerae RTX toxin gene cluster. |
Description: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Asian Fisheries Science |
Volume No.: | 22 |
Page Number: | 707-712 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/5034 |
Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
2017-05-18-PCRCloningandPartialSequencingofrtxAGeneofnon-O1-non-O139VibriocholeraeIsolatedfromGoldFishCarassiusauratusinIndia..pdf | 212 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.