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http://krishi.icar.gov.in/jspui/handle/123456789/50780Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | V Mageshwaran | en_US |
| dc.contributor.author | V Sharma | en_US |
| dc.contributor.author | M Chinnkar | en_US |
| dc.contributor.author | N Parvez | en_US |
| dc.contributor.author | V Krishnan | en_US |
| dc.date.accessioned | 2021-07-28T10:22:07Z | - |
| dc.date.available | 2021-07-28T10:22:07Z | - |
| dc.date.issued | 2018-01-01 | - |
| dc.identifier.citation | Mageshwaran V., Sharma V, M. Chinnkar, N. Parvez and V. Krishnan. 2018. Biodegradation of gossypol by mixed fungal cultures in minimal medium” Applied Biochemistry and Microbiology.54 (3): 301-308.le | en_US |
| dc.identifier.issn | ISSN 0003-6838 | - |
| dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/50780 | - |
| dc.description | Not Available | en_US |
| dc.description.abstract | The fungal cultures, namely – Pleurotus sajor-caju MTCC 1806, Saccharomyces cerevisiae MTCC 6933 and Candida tropicalis MTCC 1406 and their combinations, C. tropicalis + S. cerevisiae, P. sajor-caju + S. cerevisiae and C. tropicalis + P. sajor-caju were grown in minimal medium containing 100 ppm of gossypol as the sole carbon and energy source. The culture supernatants of C. tropicalis + S. cerevisiae and P. sajor-caju + S. cerevisiae had low residual gossypol levels of 29 and 25 ppm, respectively. In the present study, we attempted to isolate gossypol-degrading enzyme and biodegraded gossypol from the culture supernatants of C. tropicalis + S. cerevisiae and P. sajor-caju + S. cerevisiae. The specific activity of laccase in the purified enzyme extracts of the C. tropicalis + S. cerevisiae and P. sajor-caju + S. cerevisiae treated samples was 425 and 224 U/mg, respectively. In SDS-PAGE, the gossypol-degrading enzyme was revealed as 3 bands of molecular weights ranging from 45 to 66 kDa. The characterization of biodegraded gossypol by FTIR analysis showed a reduction in aldehydes (C-H) stretches in samples treated with fungi. Mass spectrometry analysis revealed that the monoisotopic mass of the biodegraded gossypol was 474 g/mol. | en_US |
| dc.description.sponsorship | National Agricultural Science Fund (NASF), New Delhi | en_US |
| dc.language.iso | English | en_US |
| dc.publisher | Pleiades Publishing | en_US |
| dc.relation.ispartofseries | Not Available; | - |
| dc.subject | biodegradation, Candida tropicalis, gossypol, Pleurotus sajor-caju, Saccharomyces cerevisiae | en_US |
| dc.title | Biodegradation of Gossypol by Mixed Fungal Cultures in Minimal Medium | en_US |
| dc.title.alternative | Not Available | en_US |
| dc.type | Article | en_US |
| dc.publication.projectcode | PHT 3026 | en_US |
| dc.publication.journalname | Applied Biochemistry and Microbiology | en_US |
| dc.publication.volumeno | 54 | en_US |
| dc.publication.pagenumber | 301-308. | en_US |
| dc.publication.divisionUnit | Not Available | en_US |
| dc.publication.sourceUrl | DOI: 10.1134/S0003683818030092 | en_US |
| dc.publication.authorAffiliation | ICAR::Central Institute for Research on Cotton Technology | en_US |
| dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
| dc.publication.naasrating | 7.02 | en_US |
| Appears in Collections: | CS-NBAIMO-Publication | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ABM - Gossypol paper- May 2018.pdf | 952.36 kB | Adobe PDF | View/Open |
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