KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/50780| Title: | Biodegradation of Gossypol by Mixed Fungal Cultures in Minimal Medium |
| Other Titles: | Not Available |
| Authors: | V Mageshwaran V Sharma M Chinnkar N Parvez V Krishnan |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Institute for Research on Cotton Technology |
| Published/ Complete Date: | 2018-01-01 |
| Project Code: | PHT 3026 |
| Keywords: | biodegradation, Candida tropicalis, gossypol, Pleurotus sajor-caju, Saccharomyces cerevisiae |
| Publisher: | Pleiades Publishing |
| Citation: | Mageshwaran V., Sharma V, M. Chinnkar, N. Parvez and V. Krishnan. 2018. Biodegradation of gossypol by mixed fungal cultures in minimal medium” Applied Biochemistry and Microbiology.54 (3): 301-308.le |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The fungal cultures, namely – Pleurotus sajor-caju MTCC 1806, Saccharomyces cerevisiae MTCC 6933 and Candida tropicalis MTCC 1406 and their combinations, C. tropicalis + S. cerevisiae, P. sajor-caju + S. cerevisiae and C. tropicalis + P. sajor-caju were grown in minimal medium containing 100 ppm of gossypol as the sole carbon and energy source. The culture supernatants of C. tropicalis + S. cerevisiae and P. sajor-caju + S. cerevisiae had low residual gossypol levels of 29 and 25 ppm, respectively. In the present study, we attempted to isolate gossypol-degrading enzyme and biodegraded gossypol from the culture supernatants of C. tropicalis + S. cerevisiae and P. sajor-caju + S. cerevisiae. The specific activity of laccase in the purified enzyme extracts of the C. tropicalis + S. cerevisiae and P. sajor-caju + S. cerevisiae treated samples was 425 and 224 U/mg, respectively. In SDS-PAGE, the gossypol-degrading enzyme was revealed as 3 bands of molecular weights ranging from 45 to 66 kDa. The characterization of biodegraded gossypol by FTIR analysis showed a reduction in aldehydes (C-H) stretches in samples treated with fungi. Mass spectrometry analysis revealed that the monoisotopic mass of the biodegraded gossypol was 474 g/mol. |
| Description: | Not Available |
| ISSN: | ISSN 0003-6838 |
| Type(s) of content: | Article |
| Sponsors: | National Agricultural Science Fund (NASF), New Delhi |
| Language: | English |
| Name of Journal: | Applied Biochemistry and Microbiology |
| NAAS Rating: | 7.02 |
| Volume No.: | 54 |
| Page Number: | 301-308. |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | DOI: 10.1134/S0003683818030092 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50780 |
| Appears in Collections: | CS-NBAIMO-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ABM - Gossypol paper- May 2018.pdf | 952.36 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.