KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/50901
Title: | Efficient and rapid method for the screening of transgenic rice plants |
Other Titles: | Not Available |
Authors: | Karikalan Jayaraman Amitha Mithra Sevanthi Sivakumar SR Pranab Kumar Mandal |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Institute for Plant Biotechnology |
Published/ Complete Date: | 1001-01-01 |
Project Code: | Not Available |
Keywords: | Molecular biology and biotechnology |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Rice is one of the most important cereals crops with great potential for improvement through biotechnological intervention. Agrobacterium-mediated transformation method for rice has been well established. In the transformation method, antibiotic resistant gene of hpt is routinely used as a powerful marker for transgenic rice plants selection. In this study, the hygromycin sensitivity test was executed on wild type Pusa Sugandh 2 (PS2) seeds, based on mesocotyl length, root development and leaf color, the most efficient optimal concentration of hygromycin 50mg/L was used for selection of the transgenic seeds and leaf assay. Wild type seedlings revealed reduction in mesocotyls lengths, root elongation and bleached leaf, as compared to transgenic seedlings on hygromycin selection media. Non-transgenic rice leaves (PS2) had phenotypic symptoms of necrosis, dark-brownish strips or bleached tips and entirely bleach out of lamina, while transgenic leaf remained green and healthy after one week of hygromycin selection. Based on the above results, a simple, rapid, inexpensive, time-saving method for effective evaluation of transgenic progeny containing hpt gene using leaf and germination assay of transformed seeds are described. This study confirms an unambiguous differentiation between transgenic and non-transgenic plants. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | International Journal of Botany Studies |
NAAS Rating: | Not Available |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50901 |
Appears in Collections: | CS-NIPB-Publication |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.