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http://krishi.icar.gov.in/jspui/handle/123456789/50963| Title: | Development of a single tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper |
| Other Titles: | Not Available |
| Authors: | A. I.Bhat S.Siju |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR-Indian Institute of Spices Research |
| Published/ Complete Date: | 2007-10-10 |
| Project Code: | DBT funded project |
| Keywords: | Black pepper, Cucumber mosaic virus, Piper yellow mottle virus, total nucleic acid isolation |
| Publisher: | Current Science Association, Bengaluru |
| Citation: | Bhat, A.I. and Siju, S. 2007. Development of a single tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper. Current Science, 93: 973-976. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Single-tube multiplex RT–PCR was developed for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus in black pepper. PCR primers designed for amplifying 650 bp from the coat protein gene of CMV and 450 bp from the open reading frame I of PYMoV were used in the reaction. The quantity of template used in mRT–PCR was found to be crucial for successful amplification. The method was found successful for the detection of both the viruses infecting black pepper in nursery and field samples. The method is rapid, reliable and requires only small tissue sample for sensitive detection. The entire procedure can aid in the rapid screening of a large number of plants for both viruses |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Current Science |
| Journal Type: | Research Journal |
| NAAS Rating: | 6.73 |
| Impact Factor: | 0.756 |
| Volume No.: | 973-976 |
| Page Number: | 973-976 |
| Name of the Division/Regional Station: | Crop Protection |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50963 |
| Appears in Collections: | HS-IISR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| multiplex PCR.pdf | 81.11 kB | Adobe PDF | View/Open |
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