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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/50963Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | A. I.Bhat | en_US |
| dc.contributor.author | S.Siju | en_US |
| dc.date.accessioned | 2021-07-29T05:33:50Z | - |
| dc.date.available | 2021-07-29T05:33:50Z | - |
| dc.date.issued | 2007-10-10 | - |
| dc.identifier.citation | Bhat, A.I. and Siju, S. 2007. Development of a single tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper. Current Science, 93: 973-976. | en_US |
| dc.identifier.issn | Not Available | - |
| dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/50963 | - |
| dc.description | Not Available | en_US |
| dc.description.abstract | Single-tube multiplex RT–PCR was developed for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus in black pepper. PCR primers designed for amplifying 650 bp from the coat protein gene of CMV and 450 bp from the open reading frame I of PYMoV were used in the reaction. The quantity of template used in mRT–PCR was found to be crucial for successful amplification. The method was found successful for the detection of both the viruses infecting black pepper in nursery and field samples. The method is rapid, reliable and requires only small tissue sample for sensitive detection. The entire procedure can aid in the rapid screening of a large number of plants for both viruses | en_US |
| dc.description.sponsorship | Not Available | en_US |
| dc.language.iso | English | en_US |
| dc.publisher | Current Science Association, Bengaluru | en_US |
| dc.relation.ispartofseries | Not Available; | - |
| dc.subject | Black pepper, Cucumber mosaic virus, Piper yellow mottle virus, total nucleic acid isolation | en_US |
| dc.title | Development of a single tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper | en_US |
| dc.title.alternative | Not Available | en_US |
| dc.type | Research Paper | en_US |
| dc.publication.projectcode | DBT funded project | en_US |
| dc.publication.journalname | Current Science | en_US |
| dc.publication.volumeno | 973-976 | en_US |
| dc.publication.pagenumber | 973-976 | en_US |
| dc.publication.divisionUnit | Crop Protection | en_US |
| dc.publication.sourceUrl | Not Available | en_US |
| dc.publication.authorAffiliation | ICAR-Indian Institute of Spices Research | en_US |
| dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
| dc.publication.journaltype | Research Journal | en_US |
| dc.publication.naasrating | 6.73 | en_US |
| dc.publication.impactfactor | 0.756 | en_US |
| Appears in Collections: | HS-IISR-Publication | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| multiplex PCR.pdf | 81.11 kB | Adobe PDF | View/Open |
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