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http://krishi.icar.gov.in/jspui/handle/123456789/51825
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jitendra K Biswal | en_US |
dc.contributor.author | Punam Bisht | en_US |
dc.contributor.author | Saravanan Subramaniam | en_US |
dc.contributor.author | Rajeev Ranjan | en_US |
dc.contributor.author | Gaurav K Sharma | en_US |
dc.contributor.author | Bramhadev Pattnaik | en_US |
dc.date.accessioned | 2021-08-01T11:55:38Z | - |
dc.date.available | 2021-08-01T11:55:38Z | - |
dc.date.issued | 2015-09-01 | - |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/51825 | - |
dc.description.abstract | Immobilized metal affinity chromatography (IMAC) allows for the efficient protein purification via metal affinity tag such as hexa-histidine (His6) sequence. To develop a new chromatography strategy for the purification and concentration of foot-and-mouth disease virus (FMDV) particles, we inserted the His6-tag at the earlier reported site in the VP1 G-H loop of the FMD virus serotype O vaccine strain IND R2/1975. Display of the His6-tag on the capsid surface, endowed the virus with an increased affinity for immobilized nickel ions. We demonstrated that the His6-tagged FMDV could be produced to high titre and purified from the infected BHK-21 cell lysates by IMAC efficiently. Further, a 1150-fold reduction in protein contaminant level and an 8400-fold reduction in DNA contaminant level were achieved in the IMAC purification of His6-tagged FMDV. Through various functional assays it has been found that the tagged virus retains its functionality and infectivity similar to the non-tagged virus. The affinity purification of the His6-tagged FMDV may offer a feasible, alternative approach to the current methods of FMDV antigen purification, concentration and process scalability. | en_US |
dc.language.iso | English | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Affinity chromatography; FMD virus; FMDV purification; His(6)-tagged FMDV; Reverse genetics. | en_US |
dc.title | Engineering foot-and-mouth disease virus serotype O IND R2/1975 for one-step purification by immobilized metal affinity chromatography | en_US |
dc.type | Journal | en_US |
dc.publication.journalname | Biologicals | en_US |
dc.publication.volumeno | 43(5) | en_US |
dc.publication.pagenumber | 390-8 | en_US |
dc.publication.sourceUrl | 10.1016/j.biologicals.2015.06.001 | en_US |
dc.publication.authorAffiliation | ICAR::Project Directorate on Foot and Mouth Disease | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | Research | en_US |
dc.publication.naasrating | 7.8 | en_US |
Appears in Collections: | AS-PDFMD-Publication |
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