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Title: | Phosphorus, protein and DNA contents of buffalo oocytes before and after in vitro maturation: effects of IGF-I |
Other Titles: | Not Available |
Authors: | Nandi S Gupta PSP JP Ravindra |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-NIANP |
Published/ Complete Date: | 2007-09-01 |
Project Code: | Not Available |
Keywords: | Animal Physiology |
Publisher: | ICAR |
Citation: | Nandi S, Gupta PSP and JP Ravindra (2007) phosphorus, protein and DNA contents of buffalo oocytes before and after in vitro maturation: effects of IGF-I. Indian journal of animal Sciences 77:818-820. |
Series/Report no.: | Not Available; |
Abstract/Description: | Studies were undertaken to determine the phosphorus, protein and DNA contents of viable buffalo oocytes before and after in vitro maturation with supplementation of different concentrations of IGF-I (0, 25, 100, 200 ng/ml) in culture medium. Oocytes were aspirated from abattoir derived buffalo ovaries. The oocytes with homogenous ooplasm and compact cumulus cell layers were preliminarily selected and stained with trypan blue to test their vitality. The concentration of 200 ng/ml of IGF-I in the maturation medium was optimum for in vitro maturation of buffalo oocytes. IGF-I stimulated in vitro oocyte maturation in a dose-dependent manner. The phosphorus, protein and DNA were significantly higher in oocytes after culture compared to those before culture in all treatments. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Journal |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Animal Sciences |
NAAS Rating: | 6.28 |
Volume No.: | 77(9) |
Page Number: | 818-820 |
Name of the Division/Regional Station: | Animal Physiology Division |
Source, DOI or any other URL: | icar.org.in |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/52418 |
Appears in Collections: | AS-NIANP-Publication |
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