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http://krishi.icar.gov.in/jspui/handle/123456789/57566| Title: | Molecular characterization and genogrouping of classical swine fever virus isolated from field outbreaks |
| Other Titles: | Not Available |
| Authors: | Chakraborty S Veeregouda BM Chandranaik BM Rathnamma D Isloor S Venkatesha MD Leena G Veeresh H Patil SS |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Karnataka Veterinary, Animal and Fisheries University, Bengaluru, Karnataka. Project Directorate on Animal Disease Monitoring and Surveillance |
| Published/ Complete Date: | 2011-04-06 |
| Project Code: | Not Available |
| Keywords: | Classical swine fever virus Genogrouping Molecular characterization Molecular outbreaks |
| Publisher: | Indian Journal of Animal Sciences |
| Citation: | Chakraborty S, Veeregouda BM, Chandranaik BM, Rathnamma D, Isloor S, Venkatesha MD, Leena G, Veeresh H and Patil SS. 2011. Molecular characterization and genogrouping of classical swine fever virus isolated from field outbreaks. Indian Journal of Animal Sciences 81:21-25. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The present study was carried out to characterize classical swine fever virus (CSFV) isolated from field outbreaks in suburban places of bengaluru, India, using molecular techniques and subsequent genogrouping of the virus. Various tissue samples from CSFV affected pigs (12) were collected and subjected to either virus isolation in PK-15 cell line, or RNA extraction. PCR amplification was carried out targeting the 5' NTR gene. Subsequent agarose gel electrophoresis yielded specific amplicons of 421 bp obtained from pooled samples of 3 pigs. The PCR purified products were sequenced and subjected to BLAST analysis and subsequently submitted to GenBank. The obtained nucleotide sequences were aligned using MegAlign programme and further subjected to analysis using MEGA 4 programme. All 3 field isolates were found to be grouped into subgroup 2.2 of group 2. |
| Description: | Not Available |
| ISSN: | 0367-8318 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Animal Sciences |
| Journal Type: | National Journal |
| NAAS Rating: | 6.28 |
| Impact Factor: | 0.28 |
| Volume No.: | 81(8) |
| Page Number: | 803-806 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://epubs.icar.org.in/ejournal/index.php/IJAnS/login?source=%2Fejournal%2Findex.php%2FIJAnS%2Farticle%2Fview%2F1275%2F3935 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/57566 |
| Appears in Collections: | AS-NIVEDI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Chakraborty et al 2011.pdf | 116.68 kB | Adobe PDF | View/Open |
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