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Title: | Maintenance of haemocytes of edible oyster, Crassostrea madrasensis (Preston) in artificial media |
Other Titles: | Not Available |
Authors: | Ittoop,Gijo George,K C Sanil,N K Sobhana,K S Nisha,P C |
ICAR Data Use Licennce: | Not Available |
Author's Affiliated institute: | Not Available |
Published/ Complete Date: | 2001 |
Project Code: | Not Available |
Keywords: | Edible oyster haemocytes Crassostrea madrasensis |
Publisher: | CMFRI/ICAR |
Citation: | Not Available |
Series/Report no.: | Not Available |
Abstract/Description: | The haemocytes of Crassostrea madrasensis were maintained in Hank's balanced salt solution (HBSS), HBSS with 10% foetal calf serum (FCS), tissue culture media M 199, and M 199 with 10% FCS. Total counts of viable haemocytes were taken at 0 hr, 24 hrs, 48 hrs, and 72 hrs. HBSS and HBSS with 10% FCS were found to maintain cell viability upto 72 hrs without much reduction in the count of viable cells. In M199 and M199 with 10% FCS, it was found that a substantial increase of small hyalinocytes occurred at 24 hrs and 48 hrs. This was followed by a steep fall in the number of viable cells at 72 hrs in both the media. The HBSS, HBSS with 10% FCS, M199 and M199 with 10% FCS were found to be good for maintaining the cells in viable state. However, only M 199 and M 199 with 10% FCS indicated multiplication of haemocytes. |
Description: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Fisheries |
Volume No.: | 48 |
Page Number: | 177-182 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | http://eprints.cmfri.org.in/131/1/Article_11.pdf |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/59602 |
Appears in Collections: | FS-CMFRI-Publication |
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