Amplification and Cloning of Full length ‘S’ Segment of Crimean-Congo Hemorrhagic Fever Virus from Hyalomma ticks infesting cattle in Gujarat

Abstract

Crimean-Congo hemorrhagic fever (CCHF) is an emerging zoonotic disease in India, first reportedfrom Gujarat, in December 2010. In the present study, CCHFV genome was detected in Hyalomma ticksinfesting cattle collected in early 2011, from Undani village, of Mehsana district of Gujarat, India underpost human CCHF outbreak surveillance activity. The full-length ‘S’ segment of CCHFV was amplifiedusing one-step RT-PCR, from total RNA isolated from these ticks. The 1673 bp amplicon was furthercloned in to pGEM®-T Easy vector and confirmed by restriction digestion. This full length ‘S’ segmentwas sequenced and sequence was submitted to NCBI GenBank database under Accession No JX051650.