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http://krishi.icar.gov.in/jspui/handle/123456789/5970| Title: | Detection of bovine viral diarrhoea virus (BVDV) in peripheral blood mononuclear cells (PBMCs) of BVDV antibody positive Indian cattle following mitogen stimulation |
| Other Titles: | Not Available |
| Authors: | Gupta, V. Mishra, N. Rajukumar, K. Behera, S. P. Dubey, S.P. |
| Published/ Complete Date: | 2014-02-01 |
| Project Code: | Not Available |
| Keywords: | Bovine viral diarrhoea BVDV PBMCs Indian cattle |
| Publisher: | Indian Council of Agricultural Research. |
| Citation: | Gupta, V., Mishra, N., Rajukumar, K., Behera, S. P., Dubey, P. (2014). Detection of bovine viral diarrhoea virus (BVDV) in peripheral blood mononuclear cells (PBMCs) of BVDV antibody positive Indian cattle following mitogen stimulation. Ind J Anim Sci. 84: 156-158. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The aim of this study was to determine the in vitro permissivity of peripheral blood mononuclear cells (PBMCs) from bovine viral diarrhea virus (BVDV)-immune field cattle to homologous and heterologous BVDVs. PBMCs from seventeen BVDV-naïve and sixteen BVDV-immune animals were infected with noncytopathic BVDV-1 or BVDV-2. The immune status of cattle was indicated by the presence of virus neutralizing antibodies, while viral load of PBMCs was determined by real-time RT-PCR. The results revealed that the PBMCs from naïve or immune animals were permissive to either BVDV-1 or BVDV-2, but the viral load was significantly higher for the naïve than for the immune animals. Furthermore, the load of homologous virus in PBMCs from immune animals was lower than that of heterologous virus. Our results provide evidence that the PBMCs from BVDV-immune cattle in field are susceptible to reinfection with homologous or heterologous BVDV, albeit to a lower extent in the former case. PMID: 24957715 |
| Description: | Not Available |
| ISSN: | 0367-8318 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Animal Sciences |
| NAAS Rating: | 6.28 |
| Volume No.: | 84 |
| Page Number: | 156-158 |
| Source, DOI or any other URL: | http://epubs.icar.org.in/ejournal/index.php/IJAnS/issue/view/1104 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/5970 |
| Appears in Collections: | AS-NIHSAD-Publication |
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