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Title: | Characterization of 14-3-3 like protein gene from C. falcatum challenged sugarcane, using RACE-PCR |
Other Titles: | Not Available |
Authors: | Rahul, P. R., V. Ganesh Kumar, R. Viswanathan, A. Ramesh Sundar and P. Malathi |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Sugarcane Breeding Institute |
Published/ Complete Date: | 2012-10-15 |
Project Code: | Not Available |
Keywords: | 14-3-3 like protein, RNA ligase, dendrogram , sugarcane disease resistance mechanism |
Publisher: | Society for Sugarcane Research and Development, Coimbatore, India |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | The present study reports the full length sequencing of 14-3-3 like protein transcript from differential display (DD)-RT-PCR. The gene was characterized using the RNA ligase mediated rapid amplification of cDNA ends (RLM-RACE-PCR) technique. A 1094 bp, 14-3-3 like protein transcript (Genbank Acc. no. HO222097) revealed the presence of conserved 14-3-3 superfamily domain in the CDD search. The ORF finder tool from the NCBI, revealed the presence of a 771 bp ORF, flanked by 86 bp 5'-UTR, and 237 bp 3'-UTR in the full length sequence. The gene product was 256 amino acids in length and the calculated molecular weight was found to be 28.8 kDa. The studies involving host-pathogen interaction, wherein the 14-3-3 plays significant role is by regulating target proteins with functions of either signalling or transcription activation or defense. The multiple sequence alignment and their dendrogram revealed that the isolated 14-3-3 was more similar to rice GF14C followed by sugarcane cluster SCBFLR1026E02.g from SUCESTdatabase. Thus the identification of 14-3-3 during the incompatible interaction between sugarcane - C. falcatum is consistent with the expression pattern of defence-related genes during the host-pathogen interactions. An earlier report had suggested that 14-3-3 proteins, together with a SnRK1 (SNF-like kinases), phosphorylate and inhibit the enzyme sucrose phosphate synthase (SPS) in vitro in sugarcane. Thus the reduced expression of 14-3-3 in mature internodes may suggest an increased accumulation of sucrose content. Thus the interplay between different components of protein regulatory mechanism suggests a complex modulatory role for different isoforms of 14-3-3s thus playing an important role in response to a specific stimulus. The present study on 14-3-3 like protein indicated a specific induction upon interaction with C. falcatum, which suggest an implication for 14-3-3 to play an important role in sugarcane disease resistance mechanism. The information on the different isoforms of the 14-3-3 like protein in sugarcane is unavailable and future studies towards greater understanding of its role and their interplay needs to be established as in other crops. |
Description: | Not Available |
ISBN: | Not Available |
Type(s) of content: | Presentation |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | 245-247 |
Name of the Division/Regional Station: | Division of crop protection |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/60609 |
Appears in Collections: | CS-SBI-Publication |
Files in This Item:
File | Description | Size | Format | |
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16. Rahul 2012 ISNPSR.pdf | 120.26 kB | Adobe PDF | View/Open |
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