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http://krishi.icar.gov.in/jspui/handle/123456789/60918
Title: | Ab initio prediction of micro-RNA like structures in sugarcane viruses and their cellular targets. |
Other Titles: | Not Available |
Authors: | Brindha S. and R. Viswanathan |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Sugarcane Breeding Institute |
Published/ Complete Date: | 2016-01-01 |
Project Code: | Not Available |
Keywords: | Sugarcane yellow leaf virus (SCYLV), Sugarcane streak mosaic virus (SCSMV), Sugarcane bacilliform virus (SCBV) , |
Publisher: | International Society of Sugar Cane Technologists |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | During a viral infection in a plant, miRNAs interplay between the host and the pathogen. Sugarcane yellow leaf virus (SCYLV), Sugarcane streak mosaic virus (SCSMV), and Sugarcane bacilliform virus (SCBV) are serious diseases affecting sugarcane productivity worldwide. As a viable strategy to utilize resistance to the viruses in sugarcane, we used computational approaches to predict virus-encoded miRNAs in these viruses. We used an in silico approach to analyse SCYLV, SCSMV and SCBV virus genome sequences. Pre-miRNAs were extracted through VMir software, and screened using web tools such as Mir Para, MiPred, Mature Pred and Mature Bayes. The potential target genes were predicted using psRNATarget. From computational tools, different viral pre-miRNA hairpin sequences and 11, 8 and 13 putative mature miRNAs were predicted from SCYLV, SCSMV and SCBV genomes, respectively. These predicted miRNAs have hybridized with numerous targets. The present study is the first computational prediction of SCYLV and SCBV encoded viral miRNA and their targets in sugarcane and other monocot ESTs. This is also the first study that has identified miRNAs in P0 (SCYLV)-ORF, which is suppressor of RNA silencing. This new findings will fine-tune our strategy of using pathogen-derived resistance in sugarcane and developing virus-resistant varieties through conventional breeding |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Proceedings |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | 29 |
Page Number: | 1786-1791 |
Name of the Division/Regional Station: | Division of crop protection |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/60918 |
Appears in Collections: | CS-SBI-Publication |
Files in This Item:
File | Description | Size | Format | |
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32.Brindha & Viswanathan 2016.pdf | 684.39 kB | Adobe PDF | View/Open |
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