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http://krishi.icar.gov.in/jspui/handle/123456789/62404
Title: | Development of a Cell Culture System From Gill Explants of the Grouper, Epinephelus malabaricus (Bloch and Shneider) |
Other Titles: | Not Available |
Authors: | Sobhana,K S George,K C Venkat Ravi,G Ittoop,Gijo Paulraj,R |
ICAR Data Use Licennce: | Not Available |
Author's Affiliated institute: | Not Available |
Published/ Complete Date: | 2009 |
Project Code: | Not Available |
Keywords: | Cell Culture System Gill Explants Grouper Epinephelus malabaricus |
Publisher: | Asian Fisheries Soceity; Malaysia |
Citation: | Not Available |
Series/Report no.: | Not Available |
Abstract/Description: | A cell culture system was developed successfully from gill explants of the Malabar grouper, Epinephelus malabaricus. Gill tissue samples aseptically excised from healthy juveniles of E. malabaricus were explanted in Leibovitz’ L-15 medium supplemented with 0.07 M NaCl and 20% fetal bovine serum (FBS). A mixture of different types of cells emerged from the explants, and these cells were observed to spread and attach to the culture flasks from the second day onwards. Confluent monolayers comprising epithelioid as well as fibroblast-like cells were formed within ten days. The cells were found to grow well at 28 + 2°C. The cell monolayers were subcultured by trypsinization and seeded into new flasks, which produced confluent monolayers comprising predominantly epithelioid-like cells in subsequent passages. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Asian Fisheries Science |
Volume No.: | 22 |
Page Number: | 541-547 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | http://eprints.cmfri.org.in/5719/1/3.pdf |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/62404 |
Appears in Collections: | FS-CMFRI-Publication |
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