Captive broodstock development, breeding and seed production of Anthid fish (family: Serranidae) Marcia's anthias, Pseudanthias marcia in recirculation aquaculture system (RAS)

Abstract

Marcia's anthias, Pseudanthias marcia Randall and Hoover, 1993, belonging to the subfamily Anthiinae (family: Serranidae) is a highly sought after marine ornamental fish mainly due to its vibrant pink shade. The present study reports the first successful captive brood stock development, spawning, and larval rearing of P. marcia. Brood stock was developed in a 5-ton recirculation aquaculture system (RAS) using 12 wild caught juveniles. After 6 months of rearing, the fish (8–9.5 cm size) started courtship behavior. Spawning occurred at 1900 to 2100 h. The eggs (mean size - 617.9 ± 14.9 μ) were transparent, pelagic, non-adhesive and with single oil globule. The eggs hatched out after an incubation period of about 14–16 h at a water temperature of 29 °C. Newly hatched larvae measured 1206.6 ± 100.02 μ in total average length and increased to 1852.9 ± 24.68 μ at 48 h post hatch (ph). Mouth opened at 48–50 h post hatch (ph) and measured 76 to 80 μ. Larval rearing trials were conducted using rotifer (L and S type), wild zooplankton, copepod nauplii, artemia nauplii and microparticulate diet. Primordial fin development started by the 10th day post-hatching (dph) (larval size ~2.9 mm) while the opercular and dorsal spines were fully formed by 15 dph (total length of the larva was 4.4 mm). Larvae metamorphosed to miniature adult shape by 32–34 dph and on 50 dph they reached the pink colored juvenile stage (42–43 mm) and at this stage, they were transferred to nursery rearing tank. Four treatments with different feed combination were tried and treatment III with wild copepod gave an average survival of 5.2 ± 1.07% and treatment IV with Parvocalanus crassirostris gave an average survival of 7.3 (±2.51) %.