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http://krishi.icar.gov.in/jspui/handle/123456789/67129
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tasok Leya | - |
dc.contributor.author | Irshad Ahmad | - |
dc.contributor.author | Rupam Sharma | - |
dc.contributor.author | Gayatri Tripathi | - |
dc.contributor.author | K. Pani Prasad | - |
dc.contributor.author | K.V.Rajendran | - |
dc.contributor.author | Megha kadam Bedikar | - |
dc.date.accessioned | 2021-11-08T06:48:56Z | - |
dc.date.available | 2021-11-08T06:48:56Z | - |
dc.date.issued | 2020-4-11 | - |
dc.identifier.citation | Leya, T., Ahmad, I., Sharma, R., Tripathi, G., Kurcheti, P.P., Rajendran, K.V., Bedekar, M.K., 2020. Bicistronic DNA vaccine macromolecule complexed with poly lactic-co-glycolic acid-chitosan nanoparticles enhanced the mucosal immunity of Labeo rohita against Edwardsiella tarda infection. Internatio | English |
dc.identifier.isbn | Not Available | English |
dc.identifier.issn | 0141-8130 | English |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/67129 | - |
dc.description | Not Available | English |
dc.description.abstract | DNA vaccine is an important tool to elicit both humoral and cellular immunity. Present study investigates mucosal immune response of Labeo rohita (15 ? 04 g) to plasmid DNA (pDNA) vaccine macromolecule complexed with nanoparticles (NPs). Poly lactic-co-glycolic acid (PLGA), Chitosan (Chit) and PLGA-Chit-NPs were synthesized by double emulsion solvent evaporation method. Synthesized NPs were complexed with pDNA (pGPD + IFN) vaccine construct. Size and zeta potential of PLGA-NPs, Chit-NPs and PLGA-Chit-NPs-pDNA complex were recorded to be 120 nm and +0.5 mV, 117 nm and +32 mV, 189 nm and +11 mV, respectively. Immunization by immersion was carried out in two groups receiving PLGA-Chit-NPs-pDNA (T1) and PLGA-NPs-pDNA (T2) respectively. After immersion, samples were collected on 0, 2, 4, 7, 15 and 30 days from mucosa-associated lymphoid tissues (MALT) for mRNA expression studies of IgM, IgD and IgZ using qRT-PCR. Significant up-regulation of the mRNA expression of IgM, IgD, and IgT were observed in MALT in immunized fish compared to control. After 30 days post-immunization fish were infected with a virulent strain of Edwardsiella tarda. The highest relative percentage survival was observed in T1 (64.7%) compared to T2. The study showed better efficiency of pDNA-PLGA-Chit-NPs compared to pDNA-PLGA-NPs for inducing adaptive mucosal immunity in fish. | English |
dc.language.iso | English | English |
dc.publisher | Not Available | English |
dc.subject | Mucosal immunity,Bicistronic DNA vaccine,Poly lactic-co-glycolic acid-chitosan nanoparticles,Labeo rohita,Edwardsiella tarda | English |
dc.title | Bicistronic DNA vaccine macromolecule complexed with poly lactic-co-glycolic acid-chitosan nanoparticles enhanced the mucosal immunity of Labeo rohita against Edwardsiella tarda infection. | en_US |
dc.type | Research Paper | English |
dc.publication.journalname | International Journal of Biological Macromolecules. | en_US |
dc.publication.submitter | From ARMS | - |
Appears in Collections: | FS-CIFE-Publication |
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