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http://krishi.icar.gov.in/jspui/handle/123456789/67932
Title: | Identification of the RNA silencing suppressor activity of Sugarcane streak mosaic virus P1 gene |
Other Titles: | Not Available |
Authors: | Bagyalakshmi, K. and R. Viswanathan |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Sugarcane Breeding Institute |
Published/ Complete Date: | 2020-09-01 |
Project Code: | Not Available |
Keywords: | Sugarcane streak mosaic virus, RNA silencing suppressor, HC-Pro, P1, Silencing assay |
Publisher: | Springer India |
Citation: | 1 |
Series/Report no.: | Not Available; |
Abstract/Description: | In the present study, P1 gene of Sugarcane streak mosaic virus (SCSMV) has been proved to govern the RNA silencing suppressor activity. Agrobacterium-mediated transient expression assay with Green Fluorescent Protein (GFP) has confirmed the RSS activity of P1 gene in the model plant Nicotiana tabaccum. The qRT-PCR analysis also confirmed the RSS activity of P1 gene of SCSMV infecting sugarcane. Further, the presence of conserved motif “WG” in the P1 protein across the 10 Indian isolates of SCSMV reiterates its role as RNA silencing suppressor of the Poacevirus. Although the Helper Component-Proteinase gene (HC-Pro) has been reported to govern the RSS activity in most of the viruses of the family Potyviridae, it does not show any such activity in the SCSCMV infecting sugarcane. The absence of “WG” motif across the 16 Indian isolates of SCSMV and low level of GFP expression in the Agrobacterium-mediated transient expression assay have clearly demonstrated lack of RSS activity in the HC-Pro gene of SCSMV. Thus, mosaic-resistant cultivars can be developed by targeting the P1 gene of SCSMV through RNAi approach. |
Description: | Not Available |
ISSN: | 2347 3584 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Virus Disease (Indian Journal of Virology) |
NAAS Rating: | 5.95 |
Impact Factor: | 1.29 |
Volume No.: | 31(3) |
Page Number: | 333-340 |
Name of the Division/Regional Station: | Division of crop protection |
Source, DOI or any other URL: | DOI: 10.1007/s13337-020-00618-7 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/67932 |
Appears in Collections: | CS-SBI-Publication |
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