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http://krishi.icar.gov.in/jspui/handle/123456789/68787| Title: | Computer program for DNA marker discovery |
| Authors: | Arunachalam, V., |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Coastal Agricultural Research Institute |
| Published/ Complete Date: | 2020-06-06 |
| Project Code: | Not Available |
| Keywords: | ISCAR marker; DNA; Priming sites; RAPD; ISSR; |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Conventional Random Amplification of Polymorphic DNA and Inter Simple Sequence Repeat molecular markers work on the random process, and their success depends on the availability of the priming sites on the sequences. Failure due to inappropriate primers can cause a researcher frustrated due to waste of chemicals and time. Failure due to inappropriate primers can cost as high US$ 100 (INR 7200) for a small experiment of 20 reactions. Thousands of such experiments are conducted using polymerase chain reactions (PCR) machines worldwide every year. The software works by following simple steps: searches for priming sites of the given primer(s) in all the target sequences, calculates priming sites of sequence(s), measures distance between priming sites, and converts best amplified primers into iSCAR primers. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Success Story |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Not Available |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/68787 |
| Appears in Collections: | NRM-CCARI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| DNA marker discovery software sucess story.pdf | 198.4 kB | Adobe PDF | View/Open |
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