KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/68787
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Arunachalam, V., | en_US |
dc.date.accessioned | 2022-01-18T10:19:11Z | - |
dc.date.available | 2022-01-18T10:19:11Z | - |
dc.date.issued | 2020-06-06 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/68787 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Conventional Random Amplification of Polymorphic DNA and Inter Simple Sequence Repeat molecular markers work on the random process, and their success depends on the availability of the priming sites on the sequences. Failure due to inappropriate primers can cause a researcher frustrated due to waste of chemicals and time. Failure due to inappropriate primers can cost as high US$ 100 (INR 7200) for a small experiment of 20 reactions. Thousands of such experiments are conducted using polymerase chain reactions (PCR) machines worldwide every year. The software works by following simple steps: searches for priming sites of the given primer(s) in all the target sequences, calculates priming sites of sequence(s), measures distance between priming sites, and converts best amplified primers into iSCAR primers. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | ISCAR marker; DNA; Priming sites; RAPD; ISSR; | en_US |
dc.title | Computer program for DNA marker discovery | en_US |
dc.type | Success Story | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Not Available | en_US |
dc.publication.volumeno | Not Available | en_US |
dc.publication.pagenumber | Not Available | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR::Central Coastal Agricultural Research Institute | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | NRM-CCARI-Publication |
Files in This Item:
File | Description | Size | Format | |
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DNA marker discovery software sucess story.pdf | 198.4 kB | Adobe PDF | View/Open |
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