Haemato-Biochemical Changes in Staphylococcal Species induced Mastitis in Mouse Model

Abstract

In this study, haemato-biochemical changes in mouse mastitis by inoculating Staphylococcus epidermidis, S. chromogenes, S. haemolyticus and S. Aureus isolated from bovine milk was studied. Blood was collected by intracardiac route at 6, 12, 24, 48, 72 and 96 h. Haematological changes noticed were: white blood cells (WBC) decreased in S. haemolyticus and S. chromogenes at 48 h; S. haemolyticus and S. aureus at 96 h. S. haemolyticus showed increased lymphocyte at 24 and 48 h and in S. aureus at 48 h. Neutrophil was decreased in S. haemolyticus and S. aureus group. Glucose increased at 6 h with three Coagulase Negative Staphylococcus (CNS) species and S. aureus. Glucose and total protein increased in S. chromogenes infected mice. Globulin levels showed decrease in S. epidermidis and S. aureus. Albumin globulin ratio showed decreasing trend in S. chromogenes and S. haemolyticus inoculated mice. Aspartate aminotransferase (AST) level increased at 6 and 12 h in all infected groups. Alanine aminotransferase increased at 12 and 48 h in S. chromogenes and S. aureus respectively. Lactate dehydrogenase (LDH) increased at 6 h in all the infected mice. Thus, three CNS species induced haemato-biochemical changes in haemoglobin, WBC, neutrophil, glucose, total protein, AST and LDH which may be used as indicators for diagnosis of CNS species mastitis in mice.