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http://krishi.icar.gov.in/jspui/handle/123456789/6900| Title: | Cytokine gene expression and pathology in mice experimentally infected with different isolates of Trypanosoma evansi |
| Other Titles: | Not Available |
| Authors: | Krishnamoorthy P Sengupta PP Das S Ligi M Shome BR Rahman H |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics |
| Published/ Complete Date: | 2016-10-01 |
| Project Code: | IXX07976 |
| Keywords: | Trypanosoma evansi, Cytokine gene expression, Pathology, Mice |
| Publisher: | Eslevier Publications |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Aim of the present study was to assess the cytokine gene expression in liver, kidney and spleen and histopathological changes in mice infected with buffalo and dog isolates of Trypanosoma evansi. Forty-four Swiss albino mice was divided into eleven groups of four mice each and injected subcutaneously with 1 × 105 trypanosomes of buffalo and dog isolate to twenty mice each, four mice served as control. Mice were examined for clinical signs, blood smear for trypanosome counts. Blood for PCR, liver, kidney, spleen, heart, lung, testis and abdominal muscle for histopathology and liver, kidney, spleen for cytokine gene expression studies, were collected. Mice showed dullness, lethargy, hunched back, sluggish movements on D4 and D5 in buffalo and dog isolate, respectively. Parasite count in blood varied between the two isolates of T. evansi. By PCR, trypanosome DNA was detected on D1 and D2 for buffalo and dog isolate, respectively. Splenomegaly was observed in mice infected with buffalo isolate but not with dog isolate. Histopathological changes were observed in liver, kidney, spleen and heart of mice but no changes in testis and abdominal muscles. Blood vessels of liver, heart, lung showed presence of trypanosomes in mice infected with buffalo isolate but not for dog isolate. Cytokine gene expression of IL-2, IL-4, IL-6, IL-12, TNF-α and IFN-γ increased in liver, kidney and spleen in both these isolates. However, the buffalo isolate exhibited pronounced increase in cytokine gene expression when compare to dog isolate of T. evansi. Anti-inflammatory cytokine gene IL-10 showed 50–60 and 10–20 folds increment in buffalo and dog isolates, respectively. This is the first report of IL-4, IL-6, IL-10 and IL-12 cytokine changes in mice infected with T. evansi. A variation in pathogenicity between buffalo and dog isolates was recorded indicating buffalo isolate of T. evansi remained more pathogenic in mice. |
| Description: | Not Available |
| ISSN: | 0014-4894 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Experimental Parasitology |
| NAAS Rating: | 7.69 |
| Volume No.: | 170 |
| Page Number: | 168-176 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1016/j.exppara.2016.09.019 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/6900 |
| Appears in Collections: | AS-NIVEDI-Publication |
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