KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/6906
Title: | A Biocompatible injectable hydrogel with potent wound healing and antibacterial properties |
Other Titles: | Not Available |
Authors: | Hoque J Prakash RG Krishnamoorthy P Shome BR Haldar J |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru ICAR::National Institute of Veterinary Epidemiology and Disease Informatics |
Published/ Complete Date: | 2017-02-16 |
Project Code: | Not Available |
Keywords: | antibacterial activity; drug-resistant bacteria; hemostatic ability; injectable bioadhesive hydrogel; surgical site infections; wound healing |
Publisher: | American Chemical Society |
Citation: | Mol. Pharmaceutics 14, 4, 1218-1230 |
Series/Report no.: | Not Available; |
Abstract/Description: | Two component injectable hydrogels that cross-link in situ have been used as noninvasive wound-filling devices, i.e., sealants. These materials carry a variety of functions at the wound sites, such as sealing leaks, ceasing unwanted bleeding, binding tissues together, and assisting in wound healing processes. However, commonly used sealants typically lack antibacterial properties. Since bacterial infection at the wound site is very common, bioadhesive materials with intrinsic antibacterial properties are urgently required. Herein, we report a biocompatible injectable hydrogel with inherent bioadhesive, antibacterial, and hemostatic capabilities suitable for wound sealing applications. The hydrogels were developed in situ from an antibacterial polymer, N-(2-hydroxypropyl)-3-trimethylammonium chitosan chloride (HTCC), and a bioadhesive polymer, polydextran aldehyde. The gels were shown to be active against both Gram-positive and Gram-negative bacteria, including drug-resistant ones such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), and β-lactam-resistant Klebsiela pneumoniae. Mechanistic studies revealed that the gels killed bacteria upon contact by disrupting the membrane integrity of the pathogen. Importantly, the gels were shown to be efficacious in preventing sepsis in a cecum ligation and puncture (CLP) model in mice. While only 12.5% of animals survived in the case of mice with punctured cecam but with no gel on the punctured area (control), 62.5% mice survived when the adhesive gel was applied to the punctured area. Furthermore, the gels were also shown to be effective in facilitating wound healing in rats and ceasing bleeding from a damaged liver in mice. Notably, the gel showed negligible toxicity toward human red blood cells (only 2–3% hemolysis) and no inflammation to the surrounding tissue upon subcutaneous implantation in mice, thus proving it as a safe and effective antibacterial sealant. |
Description: | Not Available |
ISSN: | 1543-8384 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Molecular Pharmaceutics |
NAAS Rating: | 10.32 |
Volume No.: | 14(4) |
Page Number: | 1218-1230 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | DOI: 10.1021/acs.molpharmaceut.6b01104 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/6906 |
Appears in Collections: | AS-NIVEDI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.