Relative contribution of different members of OsDREB gene family towards osmotic stress tolerance in indica and japonica ecotypes of rice

Abstract

Drought/osmotic stress is the single most production constraint in rain-fed rice cultivation. Different members of the DREB gene family are known to contribute towards osmotic stress tolerance. In this study, an attempt was made to work out their relative contribution towards osmotic stress tolerance in indica and japonica ecotypes of rice. Two genotypes (one tolerant and one susceptible) from each ecotype were grown hydroponically and the 21 days old seedlings were subjected to polyethylene glycol-induced osmotic stress (15% PEG-6000 equivalent to -3.0 bars osmotic potential). The tolerant genotypes CR143 and Moroberekan were found to have superior root traits (total root length, surface area, and volume), better plant water status, and increased total dry biomass as compared to their susceptible counterparts after 10-days of osmotic stress. We observed different members of DREB gene families were differentially induced in response to osmotic shock (1 h after stress) and osmotic stress (24 h after stress), which also differed between the two rice ecotypes. From the gene expression profiles of ten DREB genes (from both DREB1 and DREB2 families), in indica two DREB genes i.e., DREB1B and DREB1G were significantly correlated with stress tolerance indices, whereas in japonica significant correlation of five DREB (DREB1A, DREB1B, DREB1D, DREB1E and DREB2B) genes were observed. From this study, we found that only one member i.e. DREB1B showed significant correlation with drought tolerance indices in both indica and japonica ecotypes. This study provides an overview of the relative contribution of different members of DREB gene families and their association with drought/osmotic stress tolerance in rice.